Chang S M, Lin C M, Dusanic D G, Cross J H
Am J Trop Med Hyg. 1979 Sep;28(5):845-53.
Antigens in extracts of two strains of Entamoeba histolytica (HT-31 and HK-9) were studied by two-dimensional immunoelectrophoresis (2D-IEP) and 32 and 20 different antigenic components were detected, respectively. Cross-reactions between heterologous systems showed a large number of shared antigens between the two strains. Extracts were fractionated by Sephadex G-200 chromatography. Of the fractions obtained, fraction II of both strains was found to have the most reactive antigenic components. Precipitin patterns of fraction II from HT-31 and HK-9 with sera from patients showed similar 2D-IEP profiles and suggested that in this fraction antigens common to both strains exist which elicit antibody in individuals with E. histolytica infections.
通过二维免疫电泳（2D-IEP）研究了两株溶组织内阿米巴（HT-31和HK-9）提取物中的抗原，分别检测到32种和20种不同的抗原成分。异源系统之间的交叉反应表明，这两株菌之间存在大量共同抗原。提取物通过Sephadex G-200柱色谱进行分级分离。在获得的各组分中，发现两株菌的组分II具有反应性最强的抗原成分。HT-31和HK-9的组分II与患者血清的沉淀素图谱显示出相似的二维免疫电泳图谱，表明在该组分中存在两株菌共有的抗原，这些抗原可在溶组织内阿米巴感染个体中引发抗体产生。
