Marquez N S, Tamayo F G, Kumate J
Arch Invest Med (Mex). 1978;9 Suppl 1:149-54.
Entamoeba histolytica (HK-9: IMSS) trophozoites were separated from the culture medium and washed and submitted to the effects of ultrasound during 10 minutes. After 10,000 rpm x 30 centrifugation, the supernatant fluid was separated in four fractions by Sephadex G-200 gel chromatography. These fractions were lyophilized, redisolved in a lesser volume and dyalized against isotonic saline solution. Antigen-antibody precipitant reactions between each of them and two rabbit antiserums were demonstrated: anti amoeba and anti fraction A. It was verified that the antisera reacted also with the culture medium, the human seric albumin and the sera from health people and patients with invasive amebiasis. The higher antigenicity, quantitatively and qualitatively, was obtained with the fraction B; the less antigenic fraction was the fraction D. In fraction A three antigens were identified. When those proteins were filtered by a Bio Gel P4 column, two fractions were eluted: A1 and A2 which were lyophilized, redisolved and dyalized in isotonic saline solution. After this treatment, fraction A1 was capable to maintain its antigenicity against the rabbit antisera and the sera from patients with invasive amebiasis, fact by which it was considered a protein from amebal origin and not as a protenic contaminant from the culture medium.
将溶组织内阿米巴（HK - 9: IMSS）滋养体从培养基中分离出来，洗涤后进行10分钟的超声处理。经10000转/分钟×30分钟离心后，上清液通过Sephadex G - 200凝胶色谱法分离为四个组分。这些组分经冻干、在较小体积中重新溶解并对等渗盐溶液进行透析。证明了它们与两种兔抗血清之间的抗原 - 抗体沉淀反应：抗阿米巴抗血清和抗组分A抗血清。还证实这些抗血清也与培养基、人血清白蛋白以及健康人和侵袭性阿米巴病患者的血清发生反应。在定量和定性方面，组分B具有更高的抗原性；抗原性最低的组分是组分D。在组分A中鉴定出三种抗原。当这些蛋白质通过Bio Gel P4柱过滤时，洗脱得到两个组分：A1和A2，将它们冻干、重新溶解并对等渗盐溶液进行透析。经过此处理后，组分A1能够保持其针对兔抗血清和侵袭性阿米巴病患者血清的抗原性，据此认为它是源自阿米巴的蛋白质，而非培养基中的蛋白质污染物。
