Bai Qing-Xian, Zhang Xiao-Yan
Department of Hematology, State Center of Bone Marrow Transplantation, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Int J Mol Sci. 2012;13(4):4831-4838. doi: 10.3390/ijms13044831. Epub 2012 Apr 16.
Combined curcumin and PS-341 treatment has been reported to enhance cytotoxicity and minimize adverse effects through ERK and p38MAPK mechanisms in human multiple myeloma cells. However, whether JNK plays similar role in this process remains unclear. In the present study, we found combined treatment altered NF-κB p65 expressions and distributions in multiple myeloma H929 cells. Western blot analysis showed combined treatment inactivated NF-κB while activated JNK signaling. Pre-treatment with JNK inhibitor SP600125 could attenuate NF-κB inactivation and restored H929 cells' survival. These results suggested that curcumin might enhance the cytotoxicity of PS-341 by interacting with NF-κB, at least in part, through JNK mechanism.
据报道，姜黄素与PS - 341联合治疗可通过ERK和p38MAPK机制增强人多发性骨髓瘤细胞的细胞毒性并将不良反应降至最低。然而，JNK在此过程中是否发挥类似作用仍不清楚。在本研究中，我们发现联合治疗改变了多发性骨髓瘤H929细胞中NF - κB p65的表达和分布。蛋白质印迹分析表明，联合治疗使NF - κB失活，同时激活JNK信号传导。用JNK抑制剂SP600125预处理可减弱NF - κB失活并恢复H929细胞的存活率。这些结果表明，姜黄素可能至少部分地通过JNK机制与NF - κB相互作用来增强PS - 341的细胞毒性。
