Division of Neuroscience and Cell Biology, Department of Experimental and Clinical Medicine, School of Medicine, Università Politecnica delle Marche, Ancona, Italy.
PLoS One. 2012;7(5):e35232. doi: 10.1371/journal.pone.0035232. Epub 2012 May 11.
The etiology of miscarriage is often multifactorial. One major cause, immunological rejection of the fetus, has not been clearly elucidated. Our aim was to establish whether the semaphorin CD100, its natural receptor CD72, and the glycoprotein CD45, implicated in immune mechanisms, are involved in pregnancy loss by examining their placental expression with real-time PCR, immunohistochemistry and western blotting techniques.
Placenta tissue from 72 Caucasian women undergoing surgical uterine evacuation due to early spontaneous pregnancy loss between the 8(th) and 12(th) week of gestation was divided into four groups based on miscarriage number. Gestational age-matched placentas from 18 healthy women without a history of miscarriage undergoing voluntary pregnancy termination were the control group. Placenta from 6 Caesarean deliveries performed at 38-40 weeks of gestation was also studied.
CD100, CD72 and CD45 were expressed in placenta and exhibited different mRNA and protein levels in normal pregnancy and miscarriage. In particular, protein levels were highly dysregulated around 10 weeks of gestation in first and second miscarriage placentas. The CD100 soluble form was produced and immediately shed from placental tissue in all samples.
Fetal CD100, CD72 and CD45 seem to play a role in miscarriage. The present data support the involvement of the fetal immune system in pregnancy maintenance as well as failure.
流产的病因往往是多因素的。一个主要原因是胎儿的免疫排斥，但尚未明确阐明。我们的目的是通过实时 PCR、免疫组织化学和 Western blot 技术检查其胎盘表达，来确定参与妊娠丢失的信号素 CD100、其天然受体 CD72 和糖蛋白 CD45 是否涉及其中，这些物质涉及免疫机制。
72 名白人女性因 8 至 12 孕周早期自发性妊娠丢失而行手术子宫排空，根据流产次数将胎盘组织分为 4 组。18 名无流产史的健康女性因自愿终止妊娠而在孕龄匹配的胎盘作为对照组。还研究了 6 例 38-40 孕周剖宫产的胎盘。
CD100、CD72 和 CD45 在胎盘中有表达，并在正常妊娠和流产中表现出不同的 mRNA 和蛋白水平。特别是，在第一和第二次流产的胎盘中，大约在 10 孕周时蛋白水平出现高度失调。所有样本中，CD100 可溶性形式均从胎盘组织中产生并立即脱落。
胎儿 CD100、CD72 和 CD45 似乎在流产中发挥作用。本数据支持胎儿免疫系统参与妊娠维持和失败。
