Shukla S D, Coleman R, Finean J B, Michell R H
Biochem J. 1979 May 1;179(2):441-4. doi: 10.1042/bj1790441.
Glycophorin prepared by a lithium di-iodosalicylate-extraction/phenol-partition method was rich in polyphosphoinositides (phosphatidyl-myo-inositol 4-phosphate and phosphatidyl-myo-inositol 4,5-bisphosphate), but glycophorin extracted by Triton X-100 showed no such enrichment. The enrichment observed in the former preparations appeared not to be caused by pre-existing association between glycophorin and polyphosphoinositides in the human erythrocyte membrane, but to be largely a consequence of the preparative procedures.
通过二碘水杨酸锂提取/苯酚分配法制备的血型糖蛋白富含多磷酸肌醇(磷脂酰肌醇4-磷酸和磷脂酰肌醇4,5-二磷酸),但用Triton X-100提取的血型糖蛋白没有这种富集现象。在前一种制备物中观察到的富集似乎不是由人红细胞膜中血型糖蛋白与多磷酸肌醇之间预先存在的结合引起的,而是很大程度上是制备过程的结果。