Department of Breast Surgery, First Affiliated Hospital, China Medical University, No, 155 North Nanjing Street, Shenyang, Liaoning Province, 110001, China.
Diagn Pathol. 2012 May 18;7:52. doi: 10.1186/1746-1596-7-52.
As a known regulator of apoptosis, survivin has positive relationship with lymphatic metastasis in breast cancer. This study aims to detect the difference in expression between survivin and vascular endothelial growth factor-C (VEGF-C) in treated breast cancer cells and tissues, and to analyze the correlation among survivin, VEGF-C and lymphatic metastasis.
Plasmid with survivin and VEGF-C shRNA and lentivirus with survivin gene were constructed and transfected into breast cancer cell ZR-75-30. Then the expressions of the two genes were examined using western blot analysis and real-time PCR. The change of invasiveness of breast cancer cells was assessed using matrigel invasion assay. Using immunohistochemistry, the expression of survivin and VEGF-C were analyzed in 108 clinical breast cancer cases with breast cancer tissue and lymph node.
Survivin regulated the expression of VEGF-C at both protein and mRNA levels in breast cancer cells. Immunohistochemical analysis showed that the level of VEGF-C expression was significantly related with that of survivin in breast cancer tissues (p<0.05). VEGF-C was found to participate in the process of breast cancer cells invasion mediated by survivin. The co-expression of the two and the single expression of any one took significant difference in positive lymph node (p<0.05).
Survivin takes an important part in regulating the expression of VEGF-C. VEGF-C could influence the invasive ability mediated by survivin. The co-expression of survivin and VEGF-C is more statistically significant to assess lymphatic metastasis in breast cancer.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/9193530897100952.
作为凋亡的已知调节剂，存活素与乳腺癌的淋巴转移呈正相关。本研究旨在检测经处理的乳腺癌细胞和组织中存活素和血管内皮生长因子 C（VEGF-C）表达的差异，并分析存活素、VEGF-C 与淋巴转移之间的相关性。
构建带有 survivin 和 VEGF-C shRNA 的质粒和带有 survivin 基因的慢病毒，并转染乳腺癌细胞 ZR-75-30。然后使用 Western blot 分析和实时 PCR 检测这两个基因的表达。使用基质胶侵袭实验评估乳腺癌细胞侵袭能力的变化。使用免疫组织化学分析，在 108 例乳腺癌组织和淋巴结中分析 survivin 和 VEGF-C 的表达。
存活素在乳腺癌细胞中调节 VEGF-C 的蛋白和 mRNA 水平表达。免疫组织化学分析显示，乳腺癌组织中 VEGF-C 的表达水平与 survivin 显著相关（p<0.05）。发现 VEGF-C 参与了 survivin 介导的乳腺癌细胞侵袭过程。两种蛋白的共表达和任何一种蛋白的单独表达在阳性淋巴结中存在显著差异（p<0.05）。
存活素在调节 VEGF-C 的表达中起着重要作用。VEGF-C 可以影响 survivin 介导的侵袭能力。存活素和 VEGF-C 的共表达在评估乳腺癌的淋巴转移方面具有更显著的统计学意义。
本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/9193530897100952.
