口服 RG1 补充剂可增强大鼠骨骼肌对抗运动诱导的氧化应激的抗氧化防御系统。
Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles.
机构信息
Laboratory of Exercise Biochemistry, Taipei Physical Education College, Taipei City, Taiwan.
Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei City, 10462, Taiwan.
出版信息
J Int Soc Sports Nutr. 2012 May 18;9(1):23. doi: 10.1186/1550-2783-9-23.
BACKGROUND
Previous studies reported divergent results on nutraceutical actions and free radical scavenging capability of ginseng extracts. Variations in ginsenoside profile of ginseng due to different soil and cultivating season may contribute to the inconsistency. To circumvent this drawback, we assessed the effect of major ginsenoside-Rg1 (Rg1) on skeletal muscle antioxidant defense system against exhaustive exercise-induced oxidative stress.
METHODS
Forty weight-matched rats were evenly divided into control (N = 20) and Rg1 (N = 20) groups. Rg1 was orally administered at the dose of 0.1 mg/kg bodyweight per day for 10-week. After this long-term Rg1 administration, ten rats from each group performed an exhaustive swimming, and remaining rats considered as non-exercise control. Tibialis anterior (TA) muscles were surgically collected immediately after exercise along with non-exercise rats.
RESULTS
Exhaustive exercise significantly (p<0.05) increased the lipid peroxidation of control group, as evidenced by elevated malondialdehyde (MDA) levels. The increased oxidative stress after exercise was also confirmed by decreased reduced glutathione to oxidized glutathione ratio (GSH/GSSG ratio) in control rats. However, these changes were completely eliminated in Rg1 group. Catalase (CAT) and glutathione peroxidase (GPx) activities were significantly (p<0.05) increased by Rg1 in non-exercise rats, while no significant change after exercise. Nevertheless, glutathione reductase (GR) and glutathione S-transferase (GST) activities were significantly increased after exercise in Rg1 group.
CONCLUSIONS
This study provide compelling evidences that Rg1 supplementation can strengthen antioxidant defense system in skeletal muscle and completely attenuate the membrane lipid peroxidation induced by exhaustive exercise. Our findings suggest that Rg1 can use as a nutraceutical supplement to buffer the exhaustive exercise-induced oxidative stress.
背景
先前的研究报告表明，人参提取物的营养作用和自由基清除能力存在差异。由于土壤和种植季节的不同，人参中人参皂苷的分布也会发生变化，这可能是导致结果不一致的原因。为了避免这一缺陷，我们评估了主要人参皂苷-Rg1（Rg1）对骨骼肌抗氧化防御系统对抗剧烈运动引起的氧化应激的影响。
方法
40 只体重匹配的大鼠被平均分为对照组（N=20）和 Rg1 组（N=20）。Rg1 组以 0.1mg/kg 体重/天的剂量口服给药 10 周。经过长期 Rg1 给药后，每组 10 只大鼠进行剧烈游泳，其余大鼠作为非运动对照。剧烈运动后立即从运动大鼠和非运动大鼠中采集比目鱼肌（TA）。
结果
剧烈运动显著（p<0.05）增加了对照组的脂质过氧化，这表现为丙二醛（MDA）水平升高。对照组运动后氧化应激增加也得到了证实，还原型谷胱甘肽与氧化型谷胱甘肽的比值（GSH/GSSG 比值）降低。然而，这些变化在 Rg1 组完全消除。非运动大鼠中，Rg1 显著增加了过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性（p<0.05），而剧烈运动后 CAT 和 GPx 活性没有显著变化。然而，Rg1 组剧烈运动后谷胱甘肽还原酶（GR）和谷胱甘肽 S-转移酶（GST）的活性显著增加。
结论
这项研究提供了令人信服的证据，表明 Rg1 补充剂可以增强骨骼肌的抗氧化防御系统，并完全减轻剧烈运动引起的膜脂质过氧化。我们的发现表明，Rg1 可以作为一种营养补充剂，缓冲剧烈运动引起的氧化应激。
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