Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam, South Korea.
Fish Shellfish Immunol. 2012 Aug;33(2):197-203. doi: 10.1016/j.fsi.2012.04.015. Epub 2012 May 16.
Interferon gamma (IFN-γ) is a cytokine that plays a very important role in defining Th1 immune response in all vertebrates. In this study, recombinant IFN-γ (rIFN-γ) from the olive flounder (Paralichthys olivaceus) was produced in an Escherichia coli system using a pET expression vector. Stimulation of whole kidney leukocytes (immune-related cells) in vitro with the resulting rIFN-γ significantly induced the gene expression of interleukin-1β (IL-1β), signal transducer and activator of transcription 1 (STAT1), CXCL13-like chemokine (CXCL13), and IFN-γ. rIFN-γ also weakly induced the expression of IL-1β, tumor necrosis factor-α (TNF-α), CXCL13, and IFN-γ in olive flounder-derived HINAE (non-immune) cells. The effects of rIFN-γ against Edwardsiella tarda infection in vivo were assessed by intraperitoneally injecting a mixture of rIFN-γ (100 ng) and E. tarda (1 × 10(5) CFU/ml) into the olive flounder. The survival rate in the rIFN-γ-injected group was 60% compared to 0% in the group treated with E. tarda only, demonstrating that olive flounder IFN-γ is effective in reinforcing immune responses and preventing against edwardsiellosis.
干扰素 γ（IFN-γ）是一种细胞因子，在所有脊椎动物中对定义 Th1 免疫反应起着非常重要的作用。在这项研究中，使用 pET 表达载体在大肠杆菌系统中生产了来自橄榄石斑鱼（Paralichthys olivaceus）的重组 IFN-γ（rIFN-γ）。用所得的 rIFN-γ体外刺激全肾白细胞（免疫相关细胞），显著诱导白细胞介素-1β（IL-1β）、信号转导和转录激活因子 1（STAT1）、趋化因子 CXCL13 样（CXCL13）和 IFN-γ的基因表达。rIFN-γ在来自橄榄石斑鱼的 HINAE（非免疫）细胞中也弱诱导 IL-1β、肿瘤坏死因子-α（TNF-α）、CXCL13 和 IFN-γ的表达。通过向橄榄石斑鱼腹腔内注射 rIFN-γ（100ng）和 E. tarda（1×10(5)CFU/ml）混合物，评估 rIFN-γ 对爱德华氏菌感染的体内作用。与仅用 E. tarda 处理的组相比，rIFN-γ 注射组的存活率为 60%，表明橄榄石斑鱼 IFN-γ可有效增强免疫反应并预防爱德华氏菌病。
