Sysmex UF-1000i 对腹腔液流式细胞分析的分析评估。
Analytical evaluation of Sysmex UF-1000i for flow cytometric analysis of peritoneal fluid.
机构信息
Laboratorio Analisi Chimico Cliniche, AO Ospedali Riuniti Bergamo, Bergamo, Italy.
出版信息
Clin Biochem. 2012 Oct;45(15):1263-5. doi: 10.1016/j.clinbiochem.2012.05.005. Epub 2012 May 17.
OBJECTIVE
To evaluate analytical performance of Sysmex UF-1000i for peritoneal fluid analysis.
METHODS
Functional sensitivity, imprecision, linearity and comparison studies were performed on peritoneal fluids.
RESULTS
Total imprecision was 1.6-4.7%, functional sensitivity 27/μL for white blood cell (WBC) and 32/μL for total nucleated cell (TNC) count. Linearity was excellent up to 983 cell/μL, carry-over <0.2%, correlation with manual microscopy always greater than 0.992.
CONCLUSION
The instrument exhibited optimal performance at the conventional WBC diagnostic thresholds.
目的
评估 Sysmex UF-1000i 在腹腔液分析中的分析性能。
方法
对腹腔液进行了功能灵敏度、不精密度、线性和对比研究。
结果
总不精密度为 1.6-4.7%，白细胞（WBC）的功能灵敏度为 27/μL，总核细胞（TNC）计数为 32/μL。线性度优异，高达 983 个/μL，无携带污染<0.2%，与人工显微镜的相关性始终大于 0.992。
结论
该仪器在常规 WBC 诊断阈值下表现出最佳性能。
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