Gold Katrina S, Brand Andrea H
The Gurdon Institute, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2012;916:99-110. doi: 10.1007/978-1-61779-980-8_8.
In Drosophila, the central nervous system is populated by a set of asymmetrically dividing neural stem cells called neuroblasts. Neuroblasts are derived from epithelial or neuroepithelial precursors, and divide along their apico-basal axes to produce a large apical neuroblast and a smaller basal ganglion mother cell. The ganglion mother cell will divide once again to produce two post-mitotic neurons or glia. In this chapter we outline a method for labeling different types of neural precursors in the Drosophila central nervous system, followed by their extraction and processing for transcriptome analysis. This technique has allowed us to capture and compare the expression profiles of neuroblasts and neuroepithelial cells, resulting in the identification of key genes required for the regulation of self-renewal and differentiation.
在果蝇中，中枢神经系统由一组称为神经母细胞的不对称分裂神经干细胞组成。神经母细胞来源于上皮或神经上皮前体细胞，沿其顶-基轴分裂，产生一个大的顶端神经母细胞和一个较小的基底神经节母细胞。神经节母细胞会再次分裂，产生两个有丝分裂后的神经元或神经胶质细胞。在本章中，我们概述了一种在果蝇中枢神经系统中标记不同类型神经前体细胞的方法，随后对其进行提取和处理以进行转录组分析。这项技术使我们能够捕获并比较神经母细胞和神经上皮细胞的表达谱，从而鉴定出调节自我更新和分化所需的关键基因。
