Département de Biochimie, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Québec, Canada.
Database (Oxford). 2012 May 20;2012:bas025. doi: 10.1093/database/bas025. Print 2012.
Human alternative open reading frames (HAltORF) is a publicly available and searchable online database referencing putative products of out-of-frame alternative translation initiation (ATI) in human mRNAs. Out-of-frame ATI is a process by which a single mRNA encodes independent proteins, when distinct initiation codons located in different reading frames are recognized by a ribosome to initiate translation. This mechanism is largely used in viruses to increase the coding potential of small viral genomes. There is increasing evidence that out-of-frame ATI is also used in eukaryotes, including human, and may contribute to the diversity of the human proteome. HAltORF is the first web-based searchable database that allows thorough investigation in the human transcriptome of out-of-frame alternative open reading frames with a start codon located in a strong Kozak context, and are thus the more likely to be expressed. It is also the first large scale study on the human transcriptome to successfully predict the expression of out-of-frame ATI protein products that were previously discovered experimentally. HAltORF will be a useful tool for the identification of human genes with multiple coding sequences, and will help to better define and understand the complexity of the human proteome. Database URL: http://haltorf.roucoulab.com/.
人类选择性开放阅读框 (HAltORF) 是一个公开的、可在线搜索的数据库，其中引用了人类 mRNA 中推测的无框架选择性翻译起始 (ATI) 产物。无框架 ATI 是一种单个 mRNA 编码独立蛋白质的过程，当位于不同阅读框中的不同起始密码子被核糖体识别以起始翻译时，就会发生这种情况。这种机制在病毒中被广泛用于增加小病毒基因组的编码潜力。越来越多的证据表明，无框架 ATI 也存在于真核生物中，包括人类，并且可能有助于人类蛋白质组的多样性。HAltORF 是第一个基于网络的可搜索数据库，允许在具有强 Kozak 上下文的起始密码子的框架中对人类转录组中的无框架选择性开放阅读框进行彻底研究，因此更有可能表达。这也是第一个对人类转录组进行的大规模研究，成功地预测了先前通过实验发现的无框架 ATI 蛋白产物的表达。HAltORF 将是识别具有多个编码序列的人类基因的有用工具，并将有助于更好地定义和理解人类蛋白质组的复杂性。数据库网址：http://haltorf.roucoulab.com/。
