McIntyre C L, Pereira S, Moran L B, Appels R
Department of Agronomy, University of Missouri, Columbia 65211.
Genome. 1990 Oct;33(5):635-40. doi: 10.1139/g90-094.
Subcloning of a clone of the 120-bp family of rye, pSc119, has produced two extremely useful probes. pSc119.1 assays rye-specific dispersed repetitive sequence families. It is present on all seven rye chromosomes and hybridizes to the entire length of each chromosome, with the exception of some telomeres and the nucleolar organiser region. pSc119.2, in contrast, hybridizes predominantly to the telomeric regions of rye chromosomes, with some interstitial sites. Unlike pSc119.1, it assays similar repetitive sequence families in both wheat and rye chromosomes.
黑麦120bp家族的一个克隆pSc119进行亚克隆后产生了两个极其有用的探针。pSc119.1检测黑麦特异的分散重复序列家族。它存在于所有七条黑麦染色体上，能与每条染色体的全长杂交，但一些端粒和核仁组织区除外。相比之下，pSc119.2主要与黑麦染色体的端粒区域杂交，也有一些居间位点。与pSc119.1不同，它检测小麦和黑麦染色体中相似的重复序列家族。
