Cuadrado A, Jouve N
Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
Chromosome Res. 1994 Jul;2(4):331-8. doi: 10.1007/BF01552727.
Three families of highly repeated sequences from rye and the rRNA multigenes (NOR and 5S) have been mapped by FISH and C-banding, in chromosomes of triticale. The pSc119.2 probe showed interstitial hybridization in chromosome arms 1RS, 1RL, 4RL, 5RL, 6RS, 6RL, 7RS and 7RL, and is very effective for chromosome identification of rye chromosomes in triticale. This sequence also hybridizes to the 4A, 5A and the seven B-genome wheat chromosomes. Simultaneous hybridization with the pSc119.2 and pTa794 (5S rRNA) is very useful to distinguish the metacentric chromosomes 2R and 3R. The pSc74 probe appears at interstitial sites in the long arm of the most heterobrachial chromosomes (5R and 6R). The three repetitive sequences of 120 bp, 480 bp, and 610 bp hybridize to telomeric regions in rye chromosomes. Different arrangements and complex organizations consisting of arrays of three or more family sequences were found. The results demonstrate a great variation in the relative arrangement of the repetitive sequences in the telomeres of the rye chromosomes. There were quantitative differences in each cytological marker between triticale lines in both in situ labelling and C-banding, probably as the result of differences in the number and/or kind of repeat sequence.
利用荧光原位杂交（FISH）和C显带技术，已将黑麦的三个高度重复序列家族以及核糖体RNA多基因（核仁组织区和5S）定位到小黑麦的染色体上。pSc119.2探针在1RS、1RL、4RL、5RL、6RS、6RL、7RS和7RL染色体臂上显示出居间杂交信号，对小黑麦中黑麦染色体的鉴定非常有效。该序列也与4A、5A以及小麦B基因组的七条染色体杂交。同时用pSc119.2和pTa794（5S rRNA）进行杂交，对于区分中着丝粒染色体2R和3R非常有用。pSc74探针出现在大多数具异形臂染色体（5R和6R）长臂的居间位点。120bp、480bp和610bp的三个重复序列与黑麦染色体的端粒区域杂交。发现了由三个或更多家族序列阵列组成的不同排列和复杂组织形式。结果表明，黑麦染色体端粒中重复序列的相对排列存在很大差异。在原位标记和C显带中，小黑麦品系之间的每个细胞学标记都存在数量差异，这可能是重复序列数量和/或种类不同的结果。
