Cuadrado A, Jouve N
Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
Genome. 1995 Aug;38(4):795-802. doi: 10.1139/g95-101.
The molecular characterization of C-banded regions of Secale montanum Guss. by means of in situ hybridization was performed in order to provide new information about their chromosome structure relative to cultivated rye, Secale cereale L. Accurate identification of individual chromosomes was achieved using simultaneous and (or) successive fluorescent in situ hybridization (FISH) and C-banding. FISH identification was performed using total rye DNA, three highly repetitive rye DNA sequences (pSc119.2, pSc74, and pSc34), and the ribosomal RNA probes pTa71 (18S, 5.8S, and 26S rDNA) and pTa794 (5S rDNA). FISH was also used to identify the chromosome segment involved in two spontaneous translocation lines recovered from a 'Chinese Spring'--S. montanum wheat-rye addition line. FISH analysis revealed the exact translocation breakpoints and allowed the identification of the transferred rye segments. The value of this type of analysis is discussed.
为了提供关于山地黑麦（Secale montanum Guss.）C带区域相对于栽培黑麦（Secale cereale L.）染色体结构的新信息，利用原位杂交技术对山地黑麦的C带区域进行了分子特征分析。通过同时和（或）连续的荧光原位杂交（FISH）及C带技术实现了对单个染色体的准确鉴定。使用黑麦总DNA、三个高度重复的黑麦DNA序列（pSc119.2、pSc74和pSc34）以及核糖体RNA探针pTa71（18S、5.8S和26S rDNA）和pTa794（5S rDNA）进行FISH鉴定。FISH还用于鉴定从‘中国春’-山地黑麦小麦-黑麦附加系中获得的两个自发易位系中涉及的染色体片段。FISH分析揭示了确切的易位断点，并允许鉴定转移的黑麦片段。讨论了这种分析类型的价值。
