Department of Chemical and Biomolecular Engineering, National University of Singapore.
J Tissue Eng Regen Med. 2014 Jan;8(1):50-8. doi: 10.1002/term.1496. Epub 2012 May 24.
Adipose-derived stem cells (ADSCs) have great potential as a cell source for tissue engineering and regenerative medicine because they are easier to obtain, have lower donor-site morbidity and are available in larger numbers than stem cells harvested using bone marrow aspiration. Until now, little has been known about how nanotopography affects the proliferation and endothelial differentiation of ADSCs. In the present study, two nanograting substrates with a period (ridge and groove) of about 250 and 500 nm, respectively, were fabricated on quartz and their effect on ADSC fate was investigated. The results showed that proliferation of ADSCs on nanograting substrates decreased while cell attachment was not significantly affected compared to a flat substrate. Endothelial differentiation of ADSCs on both flat and nanograting substrates can be induced with vascular endothelial growth factor, as shown by immunofluorescent staining. Quantitative real-time PCR analysis showed significantly enhanced upregulation of vWF, PECAM-1 and VE-cadherin at the gene level by ADSCs on the nanograting substrates. In vitro angiogenesis assay on Matrigel showed that nanograting substrates enhanced capillary tube formation. This study highlights the beneficial influence of nanotopography on the differentiation of ADSC into endothelial cells which play an important role in vascularization.
脂肪干细胞（ADSCs）作为组织工程和再生医学的细胞来源具有巨大的潜力，因为它们更容易获得，供体部位发病率更低，并且比使用骨髓抽吸获得的干细胞数量更多。到目前为止，人们对纳米形貌如何影响 ADSC 的增殖和内皮分化知之甚少。在本研究中，在石英上制备了两个具有约 250 和 500nm 周期（脊和槽）的纳米光栅基底，并研究了它们对 ADSC 命运的影响。结果表明，与平面基底相比，ADSC 在纳米光栅基底上的增殖减少，而细胞附着没有明显受到影响。免疫荧光染色显示，在血管内皮生长因子的作用下，ADSC 可以在平面和纳米光栅基底上诱导内皮分化。定量实时 PCR 分析显示，ADSC 在纳米光栅基底上的 vWF、PECAM-1 和 VE-cadherin 基因水平的上调显著增强。在 Matrigel 上进行的体外血管生成试验表明，纳米光栅基底增强了毛细血管的形成。这项研究强调了纳米形貌对 ADSC 向内皮细胞分化的有益影响，内皮细胞在血管生成中起着重要作用。
