Departments of Dermatology and Virology and Preventive Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Japan.
Br J Dermatol. 2012 Nov;167(5):1161-4. doi: 10.1111/j.1365-2133.2012.11071.x.
Some cases of human papillomavirus (HPV) type 56 infection in Bowen disease have been reported. However, the incidence and clinical characteristics are still unclear.
To clarify the prevalence of HPV type 56-positive Bowen disease in our department and to characterize the clinical manifestations.
Sixty-eight specimens of Bowen disease were examined by polymerase chain reaction using HPV consensus primers, and the amplified products were subjected to DNA sequence analyses. Moreover, positive samples were investigated by in situ hybridization. These findings were used to clarify the clinical characteristics of HPV-positive Bowen disease.
Eight out of 68 specimens (12%) of Bowen disease were HPV-positive, of which six specimens were HPV type 56-positive. The HPV type 56-positive lesions were characterized by a longitudinal melanonychia or a deeply pigmented keratotic lesion. The remaining two specimens were genital Bowen disease in which HPV type 16 was detected. In situ hybridization demonstrated the positive cells in the upper layer of epidermis. The HPV type 56 detected in the samples of longitudinal melanonychia can be divided into at least into two types.
This study determined the prevalence of HPV type 56-positive Bowen disease. Longitudinal melanonychia is the most characteristic manifestation of HPV type 56-associated Bowen disease.
已有报道称，一些人类乳头瘤病毒（HPV）56 型感染可引起 Bowen 病。但是，其发病率和临床特征仍不清楚。
阐明本部门 HPV 56 阳性 Bowen 病的流行情况，并描述其临床表现。
应用 HPV 通用引物通过聚合酶链反应检测 68 例 Bowen 病标本，对扩增产物进行 DNA 序列分析。此外，还通过原位杂交对阳性标本进行了研究。这些发现用于阐明 HPV 阳性 Bowen 病的临床特征。
68 例 Bowen 病标本中，8 例（12%）为 HPV 阳性，其中 6 例为 HPV 56 型阳性。HPV 56 阳性病变的特征是纵向黑甲或深色素角化病变。其余 2 例为生殖器 Bowen 病，其中检测到 HPV 16 型。原位杂交显示在上皮的上层有阳性细胞。纵向黑甲中检测到的 HPV 56 可分为至少两种类型。
本研究确定了 HPV 56 阳性 Bowen 病的流行情况。纵向黑甲是 HPV 56 相关 Bowen 病最具特征性的表现。
