Peterson Stephen W
Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA.
Appl Microbiol Biotechnol. 2012 Jul;95(2):339-44. doi: 10.1007/s00253-012-4165-2. Epub 2012 May 27.
Current methods in DNA technology can detect single nucleotide polymorphisms with measurable accuracy using several different approaches appropriate for different uses. If there are even single nucleotide differences that are invariant markers of the species, we can accomplish identification through rapid DNA-based tests. The question of whether we can reliably detect and identify species of Aspergillus and Penicillium turns mainly upon the completeness of our alpha taxonomy, our species concepts, and how well the available DNA data coincide with the taxonomic diversity in the family Trichocomaceae. No single gene is yet known that is invariant within species and variable between species as would be optimal for the barcode approach. Data are published that would make an MLST approach to isolate identification possible in the most well-studied clades of Aspergillus and Penicillium.
当前的DNA技术方法可以通过几种适用于不同用途的不同方法，以可测量的准确性检测单核苷酸多态性。如果存在即使是作为物种不变标记的单核苷酸差异，我们也可以通过基于DNA的快速检测来完成鉴定。我们能否可靠地检测和鉴定曲霉属和青霉属物种的问题，主要取决于我们的α分类学的完整性、我们的物种概念，以及现有DNA数据与发菌科分类多样性的吻合程度。目前还没有一个基因已知在物种内是不变的，而在物种间是可变的，而这对于条形码方法来说是最理想的。已发表的数据将使MLST方法在曲霉属和青霉属研究最深入的进化枝中进行菌株鉴定成为可能。
