Engineering Research Center in Biomaterials, Sichuan University, Chengdu, 610064, China.
J Mater Sci Mater Med. 2012 Sep;23(9):2267-79. doi: 10.1007/s10856-012-4684-5. Epub 2012 May 26.
The network structure of a three-dimensional hydrogel scaffold dominates its performance such as mechanical strength, mass transport capacity, degradation rate and subsequent cellular behavior. The hydrogels scaffolds with interpenetrating polymeric network (IPN) structure have an advantage over the individual component gels and could simulate partly the structure of native extracellular matrix of cartilage tissue. In this study, to develop perfect cartilage tissue engineering scaffolds, IPN hydrogels of collagen/chondroitin sulfate/hyaluronan were prepared via two simultaneous processes of collagen self-assembly and cross linking polymerization of chondroitin sulfate-methacrylate (CSMA) and hyaluronic acid-methacrylate. The degradation rate, swelling performance and compressive modulus of IPN hydrogels could be adjusted by varying the degree of methacrylation of CSMA. The results of proliferation and fluorescence staining of rabbit articular chondrocytes in vitro culture demonstrated that the IPN hydrogels possessed good cytocompatibility. Furthermore, the IPN hydrogels could upregulate cartilage-specific gene expression and promote the chondrocytes secreting glycosaminoglycan and collagen II. These results suggested that IPN hydrogels might serve as promising hydrogel scaffolds for cartilage tissue engineering.
三维水凝胶支架的网络结构主导着其性能，如机械强度、传质能力、降解率和随后的细胞行为。具有互穿聚合物网络（IPN）结构的水凝胶支架优于单个成分凝胶，并且可以部分模拟软骨组织的天然细胞外基质的结构。在这项研究中，为了开发理想的软骨组织工程支架，通过胶原自组装和硫酸软骨素甲叉丙烯酰胺（CSMA）与透明质酸甲叉丙烯酰胺的交联聚合这两个同时过程制备了胶原/硫酸软骨素/透明质酸的 IPN 水凝胶。通过改变 CSMA 的甲叉化程度可以调节 IPN 水凝胶的降解速率、溶胀性能和压缩模量。体外培养兔关节软骨细胞的增殖和荧光染色结果表明，IPN 水凝胶具有良好的细胞相容性。此外，IPN 水凝胶可以上调软骨特异性基因表达，并促进软骨细胞分泌糖胺聚糖和胶原 II。这些结果表明，IPN 水凝胶可能作为软骨组织工程有前途的水凝胶支架。
