Sarker Satyajit D, Nahar Lutfun
Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, WV11LY, UK.
Methods Mol Biol. 2012;864:301-40. doi: 10.1007/978-1-61779-624-1_12.
A technique where a separation technique is coupled with an online spectroscopic detection technology is known as hyphenated technique, e.g., GC-MS, LC-PDA, LC-MS, LC-FTIR, LC-NMR, LC-NMR-MS, and CE-MS. Recent advances in hyphenated analytical techniques have remarkably widened their applications to the analysis of complex biomaterials, especially natural products. This chapter focuses on the applications of hyphenated techniques to pre-isolation and isolation of natural products, dereplication, online partial identification of compounds, chemotaxonomic studies, chemical finger-printing, quality control of herbal products, and metabolomic studies, and presents specific examples. However, a particular emphasis has been given on the hyphenated techniques that involve an LC as the separation tool.
一种将分离技术与在线光谱检测技术相结合的技术被称为联用技术，例如气相色谱-质谱联用（GC-MS）、液相色谱-光电二极管阵列检测联用（LC-PDA）、液相色谱-质谱联用（LC-MS）、液相色谱-傅里叶变换红外光谱联用（LC-FTIR）、液相色谱-核磁共振联用（LC-NMR）、液相色谱-核磁共振-质谱联用（LC-NMR-MS）以及毛细管电泳-质谱联用（CE-MS）。联用分析技术的最新进展显著拓宽了它们在复杂生物材料分析中的应用，尤其是天然产物分析。本章重点介绍联用技术在天然产物的预分离和分离、去重复、化合物的在线部分鉴定、化学分类学研究、化学指纹图谱、草药产品质量控制以及代谢组学研究中的应用，并给出具体实例。然而，特别强调了以液相色谱作为分离工具的联用技术。
