Burn Sally F
Department of Genetics and Development, Columbia University, New York, NY, USA.
Methods Mol Biol. 2012;886:241-50. doi: 10.1007/978-1-61779-851-1_21.
X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. A prerequisite is the creation or acquisition of transgenic reporter mouse lines, in which the bacterial LacZ gene has been knocked into the gene of interest or placed under the control of regulatory elements corresponding to the gene of interest. Expression is marked by a dark blue stain and can be detected at the single cell level, providing a robust visual readout of gene expression in the developing kidney. Here, we describe the methodology, applications, and limitations of this technique.
X-gal染色是一种用于检测报告基因表达的快速便捷的组织化学技术。一个前提条件是创建或获得转基因报告小鼠品系，其中细菌LacZ基因已被敲入感兴趣的基因中，或置于与感兴趣基因相对应的调控元件的控制之下。表达通过深蓝色染色标记，并且可以在单细胞水平上检测到，从而提供发育中的肾脏中基因表达的可靠视觉读数。在此，我们描述该技术的方法、应用和局限性。
