Laboratory of Edible Fungi, Institute of Technology of Agricultural Products, National Agricultural Research Foundation (NAGREF), 1 S. Venizelou Str., Lycovrysi, 14123 Athens, Greece.
Appl Biochem Biotechnol. 2012 Aug;167(7):1890-906. doi: 10.1007/s12010-012-9714-8. Epub 2012 May 26.
Volvariella volvacea strains were studied in relation with their ability to produce biomass, lipids and polysaccharides. Firstly, screening of four strains (AMLR 188, 190, 191 and 192) was performed in agar cultures, where the mycelial growth rate of the strains was measured, and in static liquid cultures, where the production of biomass, the biosynthesis of total cellular lipids and the consumption of glucose were monitored. For all strains, biomass production was significant (13-15 g l(-1)) and total lipid in dry weight (%, w/w) ranged from 3 to 12 %. Afterwards, a detailed kinetic analysis of mycelial biomass, extra- and intra- cellular polysaccharides (EPS, IPS, respectively) as well as lipid production by a V. volvacea selected strain was conducted in submerged static and agitated cultures. Maximum values of 15 g l(-1) biomass, ~1.0 g l(-1) EPS and 5.5 g l(-1) IPS were recorded. Agitation did not have severe impact on biomass, EPS and IPS production, but it increased total lipid in dry weight quantities. EPS, IPS and lipid in dry weight values decreased with time. Glucose was the major cellular carbohydrate detected. Total fatty acid analysis of cellular lipids was performed for all V. volvacea strains and linoleic acid (Δ9,12)C18:2 was predominant. Neutral lipids constituted the major fraction of cellular lipids, but their quantity decreased as fermentation proceeded. Phospholipids were the most saturated lipid fraction.
研究了草菇菌株生产生物量、脂质和多糖的能力。首先，在琼脂培养物中对 4 株菌（AMLR 188、190、191 和 192）进行了筛选，测量了菌株的菌丝生长速度，并在静态液体培养物中监测了生物量的产生、总细胞脂质的生物合成和葡萄糖的消耗。所有菌株的生物量生产都很显著（13-15 g l(-1))，干重总脂质（%，w/w）范围为 3-12%。之后，对一株草菇选择菌株在静置和搅拌的浸没静态培养中的菌丝体生物量、胞外和胞内多糖（EPS、IPS 分别）以及脂质生产进行了详细的动力学分析。记录到最大生物量为 15 g l(-1)、~1.0 g l(-1) EPS 和 5.5 g l(-1) IPS。搅拌对生物量、EPS 和 IPS 生产没有严重影响，但增加了干重总脂质的产量。EPS、IPS 和干重脂质值随时间减少。葡萄糖是检测到的主要细胞碳水化合物。对所有草菇菌株的细胞脂质进行了总脂肪酸分析，亚油酸（Δ9,12）C18:2 占优势。中性脂质构成了细胞脂质的主要部分，但随着发酵的进行，其数量减少。磷脂是最饱和的脂质部分。
