Laboratory of Edible Fungi, Institute of Technology of Agricultural Products, National Agricultural Research Foundation (NAGREF), 1 S. Venizelou Str., Lycovrysi, 14123 Athens, Greece.
Appl Biochem Biotechnol. 2012 Jun;167(3):536-51. doi: 10.1007/s12010-012-9713-9. Epub 2012 May 10.
The effect of four synthetic media containing glucose (initial concentration 30 g l(-1)) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also performed. Agitation had a positive effect on biomass production, glucose consumption and lipid biosynthesis. Media that favoured the production of biomass were not suitable for EPS biosynthesis and vice versa. Biomass values varied from ~1.0 g l(-1) (Lentinula edodes) to ~19 g l(-1) (Pleurotus ostreatus), while the highest EPS quantity achieved ranged between 1.6 and 1.8 g l(-1) (for Ganoderma lucidum and L. edodes, respectively). Quantities of total cellular lipids varied between 2.5 and 18.5 % w/w, in dry mycelial mass for the fungi tested. Lipid in dry weight values were influenced by the medium composition. Cellular lipids presented noticeable quantities of poly-unsaturated fatty acids like linoleic acid. Compared to most of the mushrooms tested, lipids of Volvariella volvacea were more saturated. The ability of several mushroom species of our study to produce in notable quantities the above-mentioned added-value compounds renders these fungi worthy for further investigations.
研究了 11 种蘑菇在静态和摇瓶培养 12 和 16 天后，四种含有葡萄糖（初始浓度 30 g/L）的合成培养基对菌丝体生长、胞外多糖（EPS）和细胞脂生产的影响。还对产生的细胞脂进行了脂肪酸分析。搅拌对生物量生产、葡萄糖消耗和脂生物合成有积极影响。有利于生物量生产的培养基不适合 EPS 生物合成，反之亦然。生物量值从1.0 g/L（香菇）到19 g/L（糙皮侧耳）不等，而获得的最高 EPS 量分别在 1.6 和 1.8 g/L（灵芝和香菇）之间。在所测试的真菌中，总细胞脂的量在干菌丝质量中在 2.5 和 18.5%w/w 之间变化。脂在干重中的含量受培养基组成的影响。细胞脂含有大量多不饱和脂肪酸，如亚油酸。与大多数测试的蘑菇相比，草菇的脂更饱和。本研究中几种蘑菇能够大量产生上述附加值化合物，这使得这些真菌值得进一步研究。
