Qin Xin-Min, Li Hui-Min, Zeng Zhen-Hua, Zeng De-Long, Guan Qing-Xin
College of Life Science, Guangxi Normal University, Guilin, Guangxi 541004, China.
Zoolog Sci. 2012 Jun;29(6):384-9. doi: 10.2108/zsj.29.384.
Black-spotted and red-spotted tokay geckos are distributed in different regions and have significant differences in morphological appearance, but have been regarded as the same species, Gekko gecko, in taxonomy. To determine whether black-spotted and red-spotted tokay geckos are genetically differentiated, we sequenced the entire mitochondrial cytochrome b gene (1147 bp) from 110 individuals of Gekko gecko collected in 11 areas including Guangxi China, Yunnan China, Vietnam, and Laos. In addition, we performed karyotypic analyses of black-spotted tokay geckos from Guangxi China and red-spotted tokay geckos from Laos. These phylogenetic analyses showed that black-spotted and red-spotted tokay geckos are divided into two branches in molecular phylogenetic trees. The average genetic distances are as follows: 0.12-0.47% among six haplotypes in the black-spotted tokay gecko group, 0.12-1.66% among five haplotypes in the red-spotted tokay gecko group, and 8.76-9.18% between the black-spotted and red-spotted tokay geckos, respectively. The karyotypic analyses showed that the karyotype formula is 2n = 38 = 8m + 2sm + 2st + 26t in red-spotted tokay geckos from Laos compared with 2n = 38 = 8m + 2sm + 28t in black-spotted tokay geckos from Guangxi China. The differences in these two kinds of karyotypes were detected on the 15th chromosome. The clear differences in genetic levels between black-spotted and red-spotted tokay geckos suggest a significant level of genetic differentiation between the two.
黑斑和红斑蛤蚧分布于不同地区，形态外观存在显著差异，但在分类学上一直被视为同一物种——蛤蚧。为了确定黑斑和红斑蛤蚧在基因上是否存在分化，我们对采自中国广西、中国云南、越南和老挝等11个地区的110只蛤蚧个体的线粒体细胞色素b基因全长（1147 bp）进行了测序。此外，我们对来自中国广西的黑斑蛤蚧和来自老挝的红斑蛤蚧进行了核型分析。这些系统发育分析表明，黑斑和红斑蛤蚧在分子系统发育树中分为两个分支。平均遗传距离如下：黑斑蛤蚧组的六个单倍型之间为0.12 - 0.47%，红斑蛤蚧组的五个单倍型之间为0.12 - 1.66%，黑斑和红斑蛤蚧之间分别为8.76 - 9.18%。核型分析表明，来自老挝的红斑蛤蚧核型公式为2n = 38 = 8m + 2sm + 2st + 26t，而来自中国广西的黑斑蛤蚧核型公式为2n = 38 = 8m + 2sm + 28t。这两种核型的差异在第15号染色体上被检测到。黑斑和红斑蛤蚧在基因水平上的明显差异表明两者之间存在显著的基因分化。
