Biotechnology Institute, School of Environment, Dalian Jiaotong University, Dalian, China.
Sci China Life Sci. 2012 May;55(5):452-66. doi: 10.1007/s11427-012-4319-6. Epub 2012 May 27.
Various active components have been extracted from the root of Polygonum cuspidatum. However, the genetic basis for their activity is virtually unknown. In this study, 25600002 short reads (2.3 Gb) of P. cuspidatum root transcriptome were obtained via Illumina HiSeq 2000 sequencing. A total of 86418 unigenes were assembled de novo and annotated. Twelve, 18, 60 and 54 unigenes were respectively mapped to the mevalonic acid (MVA), methyl-D-erythritol 4-phosphate (MEP), shikimate and resveratrol biosynthesis pathways, suggesting that they are involved in the biosynthesis of pharmaceutically important anthraquinone and resveratrol. Eighteen potential UDP-glycosyltransferase unigenes were identified as the candidates most likely to be involved in the biosynthesis of glycosides of secondary metabolites. Identification of relevant genes could be important in eventually increasing the yields of the medicinally useful constituents of the P. cuspidatum root. From the previously published transcriptome data of 19 non-model plant taxa, 1127 shared orthologs were identified and characterized. This information will be very useful for future functional, phylogenetic and evolutionary studies of these plants.
从虎杖的根部提取了各种活性成分。然而，它们的活性的遗传基础实际上是未知的。在这项研究中，通过 Illumina HiSeq 2000 测序获得了 25600002 条虎杖根转录组短读 (2.3Gb)。总共组装了 86418 条新的 unigenes，并进行了注释。有 12、18、60 和 54 个 unigenes分别映射到甲羟戊酸 (MVA)、甲基-D-赤藓醇 4-磷酸 (MEP)、莽草酸和白藜芦醇生物合成途径，表明它们参与了具有药用价值的蒽醌和白藜芦醇的生物合成。鉴定出 18 个潜在的 UDP-糖基转移酶 unigenes，它们可能是参与次生代谢物糖苷生物合成的候选基因。鉴定相关基因对于最终提高虎杖根药用成分的产量可能非常重要。从之前发表的 19 种非模式植物类群的转录组数据中，鉴定并描述了 1127 个共享的直系同源物。这些信息对于这些植物的未来功能、系统发育和进化研究将非常有用。
