García-Delgado Manuel, Navarrete-Sánchez Inés, Chamorro-Marín Virginia, Díaz-Monrové Juan Carlos, Esquivias Javier, Fernández-Mondéjar Enrique
Intensive Care Unit, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain.
ScientificWorldJournal. 2012;2012:985923. doi: 10.1100/2012/985923. Epub 2012 May 3.
This study analyses characteristics of lung injuries produced by alveolar overdistension in three animal species. Mechanical ventilation at normal tidal volume (10 mL/Kg) and high tidal volume (50 mL/Kg) was applied for 30 min in each species. Data were gathered on wet/dry weight ratio, histological score, and area of alveolar collapse. Five out of six rabbits with high tidal volume developed tension pneumothorax, and the rabbit results were therefore not included in the histological analysis. Lungs from the pigs and rats showed minimal histological lesions. Pigs ventilated with high tidal volume had significantly greater oedema, higher neutrophil infiltration, and higher percentage area of alveolar collapse than rats ventilated with high tidal volume. We conclude that rabbits are not an appropriate species for in vivo studies of alveolar overdistension due to their fragility. Although some histological lesions are observed in pigs and rats, the lesions do not appear to be relevant.
本研究分析了三种动物物种中肺泡过度扩张所导致的肺损伤特征。对每个物种分别以正常潮气量（10 mL/Kg）和高潮气量（50 mL/Kg）进行机械通气30分钟。收集了关于湿/干重比、组织学评分和肺泡萎陷面积的数据。六只接受高潮气量通气的兔子中有五只发生了张力性气胸，因此兔子的结果未纳入组织学分析。猪和大鼠的肺显示出最小的组织学损伤。与接受高潮气量通气的大鼠相比，接受高潮气量通气的猪有明显更大的水肿、更高的中性粒细胞浸润以及更高的肺泡萎陷面积百分比。我们得出结论，由于兔子的脆弱性，它们不是用于肺泡过度扩张体内研究的合适物种。虽然在猪和大鼠中观察到了一些组织学损伤，但这些损伤似乎并不相关。
