Laboratorio de Citoarquitectura y Plasticidad Neuronal, Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini", UBA-CONICET, Buenos Aires, Argentina.
Neural Plast. 2012;2012:873532. doi: 10.1155/2012/873532. Epub 2012 May 7.
Perinatal asphyxia (PA) affects the synaptic function and morphological organization. In previous works, we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia leading to long-term ubi-protein accumulation. Since F-actin is highly concentrated in dendritic spines, modifications in its organization could be related with alterations induced by hypoxia in the central nervous system (CNS). In the present study, we investigate the effects of PA on the actin cytoskeleton of hippocampal postsynaptic densities (PSD) in 4-month-old rats. PSD showed an increment in their thickness and in the level of ubiquitination. Correlative fluorescence-electron microscopy photooxidation showed a decrease in the number of F-actin-stained spines in hippocampal excitatory synapses subjected to PA. Although western blot analysis also showed a slight decrease in β-actin in PSD in PA animals, the difference was not significant. Taken together, this data suggests that long-term actin cytoskeleton might have role in PSD alterations which would be a spread phenomenon induced by PA.
围产期窒息（PA）会影响突触功能和形态组织。在之前的研究中，我们已经观察到缺氧导致大鼠新纹状体中神经元和突触发生变化，从而导致泛素蛋白的长期积累。由于 F-肌动蛋白在树突棘中高度集中，其组织的改变可能与中枢神经系统（CNS）缺氧引起的改变有关。在本研究中，我们研究了 PA 对 4 个月大的大鼠海马突触后密度（PSD）中肌动蛋白细胞骨架的影响。PSD 显示其厚度和泛素化水平增加。相关荧光电子显微镜光氧化显示，PA 处理后海马兴奋性突触中 F-肌动蛋白染色的棘突数量减少。尽管 Western blot 分析也显示 PA 动物 PSD 中的β-肌动蛋白略有减少，但差异不显著。综上所述，这些数据表明，长期肌动蛋白细胞骨架可能在 PSD 改变中起作用，这可能是 PA 引起的广泛现象。
