Peterson C M, Scott B K, Sun G Y, Sun A Y
Sansum Medical Research Foundation, Santa Barbara, California 93105.
Alcohol Clin Exp Res. 1990 Oct;14(5):717-20. doi: 10.1111/j.1530-0277.1990.tb01233.x.
Blood samples were obtained from miniature swine maintained on 0, 2, or 6 g/kg/24 hr ethanol for 8 months (N = 6 in each group). Samples from drinking pigs were taken after 8 hr of ethanol abstinence and all were coded and sent for "blinded" analysis. A fluorigenic high performance liquid chromatographic assay was used to quantify whole blood-associated acetaldehyde, hemoglobin-associated acetaldehyde, plasma-associated acetaldehyde, platelet-associated acetaldehyde, and lymphocyte-associated acetaldehyde. Detectable levels of acetaldehyde were found in each sample in both drinking and nondrinking pigs. Analysis of whole blood-associated acetaldehyde was most discriminatory in distinguishing nondrinking from drinking pigs (mean 21.4 +/- 1.0 microM for nondrinkers vs. 24.6 +/- 1.5 SD for the group consuming 2 g/kg ethanol, p = 0.001). Measurements of hemoglobin-associated acetaldehyde normalized to protein concentration (250 +/- 47 nmoles/g vs. 203 +/- 33 SD, p less than 0.05 drinking vs. nondrinking pigs) and platelet-associated acetaldehyde (0.46 0.34 vs. 0.15 +/- 0.16 nmoles/3 x 10(8) platelets, p = 0.05 drinking vs. nondrinking pigs) were also useful in discriminating drinking from nondrinking animals. Analysis of plasma-associated acetaldehyde and lymphocyte-associated acetaldehyde were not useful as markers of ethanol consumption.(ABSTRACT TRUNCATED AT 250 WORDS)
从维持8个月每日摄入0、2或6克/千克乙醇的小型猪身上采集血样（每组n = 6）。饮用乙醇的猪的血样在戒断乙醇8小时后采集，所有样本均编码并送去进行“盲法”分析。采用荧光高效液相色谱法对全血相关乙醛、血红蛋白相关乙醛、血浆相关乙醛、血小板相关乙醛和淋巴细胞相关乙醛进行定量。在饮用和未饮用乙醇的猪的每个样本中均检测到可检测水平的乙醛。全血相关乙醛分析在区分未饮用乙醇和饮用乙醇的猪方面最具鉴别力（未饮用者平均为21.4±1.0微摩尔，而摄入2克/千克乙醇组为24.6±1.5标准差，p = 0.001）。血红蛋白相关乙醛与蛋白质浓度归一化后的测量值（饮用乙醇与未饮用乙醇的猪相比，分别为250±47纳摩尔/克与203±33标准差，p＜0.05）以及血小板相关乙醛（分别为0.46±0.34与0.15±0.16纳摩尔/3×10⁸个血小板，p = 0.05）在区分饮用乙醇和未饮用乙醇的动物方面也很有用。血浆相关乙醛和淋巴细胞相关乙醛分析作为乙醇摄入的标志物并无用处。（摘要截断于250字）
