Peterson C M, Scott B K, McLaughlin S D
Sansum Medical Research Foundation, Santa Barbara, CA 93105.
Alcohol. 1991 Jan-Feb;8(1):35-8. doi: 10.1016/0741-8329(91)91216-o.
A study was undertaken in C57BL mice to evaluate the effect of gender on whole blood associated acetaldehyde following exposure to ethanol in the drinking water (10% v/v). Whole blood associated acetaldehyde (WBAA) was measured from capillary blood samples using a fluorigenic high performance chromatographic assay on days 0, 7, 15 and 27. Ethanol consumption did not impair growth of either male or female mice when compared to controls. Following administration of ethanol, WBAA increased in both male and female mice but marked gender differences were apparent. Female mice consumed more fluid relative to body weight than males (155 +/- 27 S.D. vs. 124 +/- 19 ml/kg/day, p less than 0.001), but had lower mean WBAA levels during the four weeks of ethanol administration (137 +/- 37 vs. 318 +/- 66 nmol/g hemoglobin, p less than 0.001). WBAA levels in male mice were stable over the course of the experiment. Female mice were found to have peak WBAA levels on day seven after which time levels decreased significantly. These experiments emphasize gender differences in ethanol metabolism as well as the need to establish norms based on gender for assays of ethanol consumption which use acetaldehyde adducts with blood proteins.
在C57BL小鼠中开展了一项研究，以评估性别对饮用含乙醇（10% v/v）水后全血中乙醛含量的影响。在第0、7、15和27天，使用荧光高效色谱分析法从毛细血管血样中测量全血相关乙醛（WBAA）。与对照组相比，乙醇摄入并未损害雄性或雌性小鼠的生长。给予乙醇后，雄性和雌性小鼠的WBAA均升高，但存在明显的性别差异。相对于体重，雌性小鼠比雄性小鼠消耗更多液体（155±27标准差 vs. 124±19 ml/kg/天，p<0.001），但在乙醇给药的四周内平均WBAA水平较低（137±37 vs. 318±66 nmol/g血红蛋白，p<0.001）。在实验过程中，雄性小鼠的WBAA水平保持稳定。发现雌性小鼠在第7天WBAA水平达到峰值，此后水平显著下降。这些实验强调了乙醇代谢中的性别差异，以及在使用乙醛与血液蛋白加合物进行乙醇消耗测定时，需要根据性别建立规范。
