Peterson C M, Scott B K
Sansum Medical Research Foundation, Santa Barbara, California 93105.
Alcohol Clin Exp Res. 1989 Dec;13(6):845-8. doi: 10.1111/j.1530-0277.1989.tb00435.x.
Thirty C57BI mice were randomized into two groups. Group 1 served as controls while Group 2 was given 10% V/V ethanol with the drinking water. Whole blood- associated acetaldehyde (WBAA) was measured on capillary blood samples using a fluorigenic high performance chromatographic assay. WBAA peaked at Day 2. A stable mean plateau of 263 +/- 71 SD with a range of 160-400 nmoles/g hemoglobin WBAA was found in the group consuming ethanol compared with 122 +/- 17 SD and a range of 88-150 nmoles/g hemoglobin for controls (p less than 0.001). When ethanol was discontinued, levels of WBAA declined and became similar to those of controls by 9 days following cessation of ethanol. The quantitative difference between ethanol-consuming and control animals and also the rapid rise of whole blood-associated acetaldehyde and the relatively slow decline following cessation of ethanol intake indicate that such a test might be a useful monitor of drinking behavior.
30只C57BI小鼠被随机分为两组。第1组作为对照组，而第2组的饮用水中加入10%（体积/体积）的乙醇。使用荧光高效色谱分析法对毛细血管血样中的全血相关乙醛（WBAA）进行测量。WBAA在第2天达到峰值。与对照组的122±17标准差（范围为88 - 150纳摩尔/克血红蛋白）相比，饮用乙醇的组中发现WBAA的稳定平均平台为263±71标准差（范围为160 - 400纳摩尔/克血红蛋白）（p < 0.001）。当停止给予乙醇时，WBAA水平下降，在停止乙醇摄入后9天，其水平变得与对照组相似。饮用乙醇的动物与对照动物之间的定量差异，以及全血相关乙醛的快速上升和乙醇摄入停止后相对缓慢的下降表明，这样的测试可能是饮酒行为的一个有用监测指标。
