Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Vet Q. 2012;32(1):17-23. doi: 10.1080/01652176.2012.689117.
Increased proteolytic cleavage of serum amyloid A (SAA) may potentially contribute to the development of AA amyloid deposition.
To study the possible relationship between amyloid artropathy and expression of SAA and some serum amino acids.
Values of 15 serum amino acids and SAA were investigated in chickens with experimentally induced amyloid arthropathy. Thirty-four, 5-week-old chicks were allocated into two groups: one group was injected intra-articularly with 0.25 mL complete Freund's adjuvant at the left tibio-metatarsal joint to induce amyloid arthropathy, whereas the other group served as control. All pullets were necropsied 13 weeks after injection. Collected tissue samples were examined histopathologically. Blood samples were collected and SAA concentrations were measured with enzyme-linked immunosorbent assay. High-performance liquid chromatography was used to assess the amino acid concentrations in serum.
Amyloid accumulation in joints occurred only in the experimental group (89%). SAA concentrations of 166 ± 17 and 423 ± 39 (SD) ng/mL were found in the control and experimental groups, respectively (p < 0.001). In the experimental group, an increase was observed in all examined amino acid concentrations except for citrulline. The most significant (p < 0.001) increases were noticed in serine (from 159 ± 15 to 360 ± 29 µmol/L), glycine (from 151 ± 20 to 279 ± 16 µmol/L), isoleucine (from 48 ± 2 to 80 ± 6 µmol/L), and phenylalanine (from 49 ± 2 to 90 ± 3 µmol/L).
The results of this study suggest that there is a positive correlation between some serum amino acid values, especially serine, glycine, isoleucine, and phenylalanine, and the high concentrations of SAA in chickens with amyloid arthropathy.
血清淀粉样蛋白 A（SAA）的蛋白水解裂解增加可能有助于 AA 淀粉样蛋白沉积的发展。
研究淀粉样关节病与 SAA 和一些血清氨基酸表达之间的可能关系。
研究了 34 只 5 周龄小鸡，将其分为两组：一组小鸡在左胫跗关节内注射 0.25mL 完全弗氏佐剂以诱导淀粉样关节病，另一组作为对照组。注射后 13 周，所有小鸡均进行尸检。收集组织样本进行组织病理学检查。采集血样，用酶联免疫吸附试验测量 SAA 浓度。采用高效液相色谱法测定血清中氨基酸浓度。
仅在实验组（89%）中观察到关节淀粉样物质的积累。对照组和实验组的 SAA 浓度分别为 166±17 和 423±39（SD）ng/mL（p<0.001）。在实验组中，除瓜氨酸外，所有检测到的氨基酸浓度均增加。增加最显著（p<0.001）的是丝氨酸（从 159±15 增加到 360±29µmol/L）、甘氨酸（从 151±20 增加到 279±16µmol/L）、异亮氨酸（从 48±2 增加到 80±6µmol/L）和苯丙氨酸（从 49±2 增加到 90±3µmol/L）。
本研究结果表明，在患有淀粉样关节病的鸡中，某些血清氨基酸值，尤其是丝氨酸、甘氨酸、异亮氨酸和苯丙氨酸与 SAA 浓度之间存在正相关关系。
