Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Clinical Science, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Tehran, Iran.
PLoS One. 2020 Feb 11;15(2):e0229009. doi: 10.1371/journal.pone.0229009. eCollection 2020.
Broiler chickens experience an acute-phase response (APR) through vaccination, which reflects the innate immunity and stress related to immunization. It is also considered that APR can modulate adaptive immunity and response to infection. As biomarkers for APR, assessing the acute-phase proteins (APPs) function and their levels in response to immunization is of great value for vaccine design, development and administration. In this study, the heterophils/lymphocyte (H/L) ratio and the level of APPs was evaluated in broilers with three different Newcastle disease (ND) vaccination regimens. Inactivated ND vaccine (IND) was administered by the intramuscular route. Live attenuated strains, Lasota and Vitapest, was administered by ocular routes. H/L ratio, serum amyloid A (SAA) and alpha-1 acid glycoprotein (AGP) were measured before and after two rounds of vaccination on days 10 and 21. In a comparison between the three vaccines, H/L ratio in IND group significantly increased to 3 fold (1.48 ± 0.41) after the first vaccination while the Lasota and Vitapest showed a milder response. The concentration of SAA increased after 24h by 1.8-fold in IND group (0.116 ± 0.015 mg/L) and 2-fold in Lasota group (0.14 ± 0.002 mg/L). Significant changes were found in Vitapest group after 48h post vaccination (0.113 ± 0.016 mg/L). Elevation pattern of AGP, 24 hours after first vaccination in IND (3.5-fold) and Vitapest (2.5-fold) was different from Lasota in which the peak was reached after 48 hours (2.9-fold). Except for IND group, no significant changes in SAA and AGP concentrations were detected after the second vaccination. A significant positive correlation between SAA values at day 22 and HI titers at day 28 (r = 0.998, P≤0. 0.005) was found. According to these results, different types of ND vaccines can cause different patterns of acute phase responses. Assessment of stress and level of acute-phase proteins can be used for prediction of immune response outcomes in vaccine design and development.
肉鸡在接种疫苗时会经历急性期反应 (APR)，这反映了其与免疫相关的固有免疫和应激反应。也有人认为 APR 可以调节适应性免疫和对感染的反应。作为 APR 的生物标志物，评估急性相蛋白 (APP) 的功能及其对免疫的反应水平对于疫苗的设计、开发和管理具有重要意义。在这项研究中，评估了三种不同的新城疫 (ND) 疫苗接种方案对肉鸡的异嗜细胞/淋巴细胞 (H/L) 比值和 APP 水平的影响。通过肌肉内途径给予灭活 ND 疫苗 (IND)。通过眼部途径给予活减毒株 Lasota 和 Vitapest。在第 10 天和第 21 天进行两轮疫苗接种前后，测量 H/L 比值、血清淀粉样蛋白 A (SAA) 和α-1 酸性糖蛋白 (AGP)。在三种疫苗的比较中，IND 组在第一次接种后 H/L 比值显著增加了 3 倍（1.48 ± 0.41），而 Lasota 和 Vitapest 则反应较轻。IND 组接种后 24 小时 SAA 浓度增加了 1.8 倍（0.116 ± 0.015 mg/L），Lasota 组增加了 2 倍（0.14 ± 0.002 mg/L）。Vitapest 组在接种后 48 小时后发现明显变化（0.113 ± 0.016 mg/L）。IND（3.5 倍）和 Vitapest（2.5 倍）在第一次接种后 24 小时，AGP 的升高模式与 Lasota 不同，后者在 48 小时后达到高峰（2.9 倍）。除了 IND 组外，第二次接种后 SAA 和 AGP 浓度没有明显变化。第 22 天 SAA 值与第 28 天 HI 滴度之间存在显著正相关（r = 0.998，P≤0.005）。根据这些结果，不同类型的 ND 疫苗可能导致不同的急性期反应模式。应激和急性期蛋白水平的评估可用于预测疫苗设计和开发中的免疫反应结果。
