Huang Ping, Gao Jing-wen, Shi Zhan, Zou Jia-li, Lu Ya-song, Yuan Yue-mei, Yao Mei-cun
Laboratory of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
Bioanalysis. 2012 Jun;4(10):1205-13. doi: 10.4155/bio.12.81.
Dahuang Huanglian Xiexin Decoction (DHXD) is a classical formula in traditional Chinese medicines. In this study, a novel UPLC-MS/MS method was developed for the simultaneous quantification of rhein, emodin, berberine and baicalin, the major bioactive compounds of DHXD in rat plasma.
The method possessed high sensitivity and ultrashort analysis time (7 min). Linearity, accuracy, precision and extraction recovery of four analytes were all satisfactory. The method was then successfully applied in a pharmacokinetic study of four bioactive components after a single oral administration of DHXD extract to rats.
The method was applicable for simultaneous bioanalysis of rhein, emodin, berberine and baicalin.
大黄黄连泻心汤是中药中的经典方剂。本研究建立了一种新型超高效液相色谱-串联质谱法，用于同时定量大鼠血浆中大黄黄连泻心汤的主要生物活性成分大黄酸、大黄素、小檗碱和黄芩苷。
该方法具有高灵敏度和超短分析时间（7分钟）。四种分析物的线性、准确度、精密度和提取回收率均令人满意。该方法随后成功应用于大鼠单次口服大黄黄连泻心汤提取物后四种生物活性成分的药代动力学研究。
该方法适用于大黄酸、大黄素、小檗碱和黄芩苷的同时生物分析。
