Proteomics Research Group, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa.
J Proteomics. 2012 Jul 16;75(13):4139-50. doi: 10.1016/j.jprot.2012.05.038. Epub 2012 May 29.
Sorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular mechanisms of drought and salt stress tolerance in cereals. In this study, seeds of a sweet sorghum variety, MN1618, were planted and grown on solid MS growth medium with or without 100mM NaCl. Heat shock protein expression immunoblotting assays demonstrated that this salt treatment induced stress within natural physiological parameters for our experimental material. 2D PAGE in combination with MS/MS proteomics techniques were used to separate, visualise and identify salinity stress responsive proteins in young sorghum leaves. Out of 281 Coomassie stainable spots, 118 showed statistically significant responses (p<0.05) to salt stress treatments. Of the 118 spots, 79 were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and of these, 55 were positively identified. Identified proteins were divided into six functional categories including both known and novel/putative stress responsive proteins. Molecular and physiological functions of some of our proteins of interest are currently under investigation via bioinformatic and molecular biology approaches.
高粱，一种耐旱的谷类作物，不仅是半干旱/干旱地区的重要食物来源，也是研究和更好地了解谷类作物耐旱和耐盐胁迫分子机制的潜在模式生物。在这项研究中，MN1618 甜高粱品种的种子种植在含有或不含有 100mM NaCl 的固体 MS 生长培养基上。免疫印迹分析表明，这种盐处理在我们实验材料的自然生理参数范围内诱导了应激。2D PAGE 与 MS/MS 蛋白质组学技术结合，用于分离、可视化和鉴定幼叶中的盐胁迫响应蛋白。在 281 个考马斯亮蓝染色斑点中，有 118 个对盐胁迫处理表现出统计学上显著的响应（p<0.05）。在这 118 个斑点中，由于其良好的分辨率和丰度水平，选择了 79 个进行串联质谱鉴定，其中 55 个被鉴定为阳性。鉴定的蛋白质分为六个功能类别，包括已知和新型/假定的应激响应蛋白。一些感兴趣的蛋白质的分子和生理功能目前正在通过生物信息学和分子生物学方法进行研究。
