Gobeli S, Berset C, Burgener I, Perreten V
Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland.
Schweiz Arch Tierheilkd. 2012 Jun;154(6):247-50. doi: 10.1024/0036-7281/a000340.
Fifty Clostridium perfringens strains were isolated from individual dogs with acute diarrhoea that were not given antibiotics. Toxin types and minimal inhibitory concentrations of 15 antibiotics were determined for each of them. All strains harboured the alpha-toxin gene, 12 of them had both the alpha- and entero-toxin gene and 5 had both the alpha- and beta2-toxin gene. Eighteen percent of the isolates showed resistance to tetracycline and 54 % showed decreased susceptibility to metronidazole which is one of the most frequently used antibiotics in the treatment of canine diarrhoea. Apart from that, all isolates were susceptible to the remaining antibiotics tested. These findings lead to the conclusion that despite a general susceptibility to antibiotics in C. perfringens, resistance is developing in isolates from dogs. Therefore, careful identification of the pathogenic agent and antibiotic susceptibility testing should be performed prior to therapy in order to minimise further selection of antibiotic resistance.
从患有急性腹泻且未使用抗生素的犬只个体中分离出50株产气荚膜梭菌菌株。测定了每株菌株的毒素类型和15种抗生素的最低抑菌浓度。所有菌株均携带α毒素基因，其中12株同时具有α毒素基因和肠毒素基因，5株同时具有α毒素基因和β2毒素基因。18%的分离株对四环素耐药，54%的分离株对甲硝唑的敏感性降低，甲硝唑是治疗犬腹泻最常用的抗生素之一。除此之外，所有分离株对其余测试抗生素均敏感。这些发现得出的结论是，尽管产气荚膜梭菌总体上对抗生素敏感，但来自犬只的分离株中正在产生耐药性。因此，在治疗前应仔细鉴定病原体并进行抗生素敏感性测试，以尽量减少抗生素耐药性的进一步选择。
