自身免疫性MRL/Mp-lpr/lpr小鼠中针对聚（ADP-核糖）的天然抗体。
Naturally occurring antibodies to poly(ADP-ribose) in autoimmune MRL/Mp-lpr/lpr mice.
作者信息
Kanai Y, Fujiwara M
出版信息
Clin Exp Immunol. 1985 Jan;59(1):132-8.
The presence of antibodies to poly(ADP-ribose) was demonstrated in the sera of MRL/Mp-lpr/lpr (MRL/l) mice by an enzyme linked immunosorbent assay (ELISA). Antibody to poly(ADP-ribose) was strongly inhibited by single stranded DNA (ssDNA) and poly(ADP-ribose), and less by double stranded DNA (dsDNA). Affinity purified anti-poly(ADP-ribose) antibodies bound more with immobilized ssDNA than with poly(ADP-ribose), and were significantly inhibited by soluble ssDNA, although poly(ADP-ribose) was the best soluble inhibitor. On the contrary, affinity purified anti-ssDNA antibodies bound best with ssDNA and significantly but less with poly(ADP-ribose); however, they were scarcely inhibited by poly(ADP-ribose). These results suggest that similar antigenic determinants exist in poly(ADP-ribose) and ssDNA. It is conceivable, however, at the present moment that 'naturally occurring antibodies to poly(ADP-ribose)' in MRL/l mice are subpopulations of anti-ssDNA antibodies that react equally well with poly(ADP-ribose) and ssDNA.
通过酶联免疫吸附测定（ELISA）在MRL/Mp-lpr/lpr（MRL/l）小鼠血清中证实了抗聚（ADP-核糖）抗体的存在。抗聚（ADP-核糖）抗体受到单链DNA（ssDNA）和聚（ADP-核糖）的强烈抑制，而受到双链DNA（dsDNA）的抑制较弱。亲和纯化的抗聚（ADP-核糖）抗体与固定化的ssDNA结合比与聚（ADP-核糖）结合更多，并且被可溶性ssDNA显著抑制，尽管聚（ADP-核糖）是最佳的可溶性抑制剂。相反，亲和纯化的抗ssDNA抗体与ssDNA结合最佳，与聚（ADP-核糖）结合显著但较少；然而，它们几乎不受聚（ADP-核糖）的抑制。这些结果表明聚（ADP-核糖）和ssDNA中存在相似的抗原决定簇。然而，目前可以想象，MRL/l小鼠中“天然存在的抗聚（ADP-核糖）抗体”是与聚（ADP-核糖）和ssDNA反应同样良好的抗ssDNA抗体亚群。
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