Chou M J, Lee S L, Chen T Y, Tsay G J
Department of Internal Medicine, Chung Shan Medical and Dental College, Taichung, Taiwan.
Ann Rheum Dis. 1995 Feb;54(2):148-51. doi: 10.1136/ard.54.2.148.
To study the specific autoantibodies against nuclear antigens in patients with primary biliary cirrhosis (PBC).
Sera from 21 patients with PBC were tested for antinuclear antibody (ANA) by indirect immunofluorescence on human epithelial (HEp)-2 cells, and for antibodies to various nuclear antigens by enzyme linked immunosorbent assay (ELISA) using different purified proteins or recombinant proteins as antigens.
ANA detected in 10 of 21 patients (48%) with PBC included five anti-centromere antibody (ACA), two speckled, two homogeneous and one nuclear dot staining. ACA were present in 24% of PBC patients. By ELISA, anti-histone antibodies were detected in 81% of PBC patients, anti-ssDNA antibodies in 71% and anti-dsDNA in 10%, anti-topoisomerase-1 antibodies in 24%, anti-Sm/RNP antibodies in 24%, anti-La-48(SS-A) antibodies in 21%, and anti-Ro-60(SS-A) and anti-Ro-52(SS-A) antibodies in 30% and 25%, respectively.
The high frequencies of various antibodies directed against intracellular proteins and nucleic acids in patients with PBC suggests that PBC is a multisystem autoimmune disease which is similar to other systemic autoimmune diseases.
研究原发性胆汁性肝硬化（PBC）患者针对核抗原的特异性自身抗体。
采用间接免疫荧光法在人上皮（HEp）-2细胞上检测21例PBC患者血清中的抗核抗体（ANA），并以不同的纯化蛋白或重组蛋白作为抗原，通过酶联免疫吸附测定（ELISA）检测针对各种核抗原的抗体。
21例PBC患者中有10例（48%）检测到ANA，其中包括5例抗着丝点抗体（ACA）、2例斑点型、2例均质型和1例核点型染色。24%的PBC患者存在ACA。通过ELISA检测，81%的PBC患者检测到抗组蛋白抗体，71%检测到抗单链DNA抗体，10%检测到抗双链DNA抗体，24%检测到抗拓扑异构酶-1抗体，24%检测到抗Sm/RNP抗体，21%检测到抗La-48（SS-A）抗体，30%和25%的患者分别检测到抗Ro-60（SS-A）和抗Ro-52（SS-A）抗体。
PBC患者中针对细胞内蛋白质和核酸的各种抗体的高频率出现表明，PBC是一种多系统自身免疫性疾病，与其他系统性自身免疫性疾病相似。
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