Chulalaksananukul W, Condoret J S, Delorme P, Willemot R M
Département de Génie Biochimique et Alimentaire, UA-CNRS 544, Institut National des Sciences Appliquées, Toulouse, France.
FEBS Lett. 1990 Dec 10;276(1-2):181-4. doi: 10.1016/0014-5793(90)80537-s.
The kinetic of the esterification of oleic acid by ethanol catalyzed by immobilized lipase of Mucor miehei in n-hexane as a solvent has been completely studied. The kinetics of the reaction are suggested to agree with a Ping-Pong Bi Bi mechanism in which only inhibition by excess of ethanol has been identified. Values of all apparent kinetic parameters were computed. No evidence of any significant external diffusional limitation which could account for these values has been detected. Optimization of water content through distribution ratio of water between solvent and support was examined.
以正己烷为溶剂，对米黑根毛霉固定化脂肪酶催化乙醇与油酸的酯化动力学进行了全面研究。该反应动力学符合乒乓双双机制，其中仅发现过量乙醇存在抑制作用。计算了所有表观动力学参数的值。未检测到任何可解释这些值的显著外部扩散限制的证据。通过考察溶剂与载体之间水的分配比来优化含水量。
