Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India.
Res Vet Sci. 2012 Dec;93(3):1531-6. doi: 10.1016/j.rvsc.2012.04.013. Epub 2012 May 31.
Bluetongue is an economically important viral disease of small ruminants. The present/current diagnostic kits and methods to diagnose BTV are laborious, time consuming and expensive. In the present study, we have attempted to develop a novel approach to detect BTV antibodies in sera that in future can be harnessed for developing a pen side diagnostic test. Briefly, we identified the immunodominant regions of the VP7 protein of BTV and synthesized them in the multiple antigenic peptide (MAP) format with cysteine at C-terminal of the lysine mosaic, which elicited highly ordered conformation as well as ELISA reactivity. Finally, we coated the MAP peptides on the gold nanoparticles that can be used to detect BTV specific antibodies in the sera using a spot test.
蓝舌病是一种对小反刍动物具有重要经济意义的病毒性疾病。目前/当前用于诊断 BTV 的诊断试剂盒和方法既繁琐耗时又昂贵。在本研究中，我们试图开发一种新的方法来检测血清中的 BTV 抗体，该方法未来可用于开发一种笔式诊断测试。简而言之，我们鉴定了 BTV VP7 蛋白的免疫显性区域，并以多抗原肽（MAP）的形式与赖氨酸镶嵌的 C 末端的半胱氨酸合成，从而激发了高度有序的构象和 ELISA 反应性。最后，我们将 MAP 肽涂覆在金纳米颗粒上，可使用点测试来检测血清中的 BTV 特异性抗体。
