表达蓝舌病病毒蛋白的重组山羊痘病毒：小反刍动物免疫反应及保护作用评估
Recombinant capripoxviruses expressing proteins of bluetongue virus: evaluation of immune responses and protection in small ruminants.
作者信息
Perrin Aurélie, Albina Emmanuel, Bréard Emmanuel, Sailleau Corinne, Promé Sylvie, Grillet Colette, Kwiatek Olivier, Russo Pierre, Thiéry Richard, Zientara Stephan, Cêtre-Sossah Catherine
机构信息
CIRAD-Département BioS, UPR15, TA A-15, Campus International de Baillarguet, 34398 Cedex 5 Montpellier, France.
出版信息
Vaccine. 2007 Sep 17;25(37-38):6774-83. doi: 10.1016/j.vaccine.2007.06.052. Epub 2007 Jul 16.
The development of recombinant capripoxviruses for protective immunization of ruminants against bluetongue virus (BTV) infection is described. Sheep (n=11) and goats (n=4) were immunized with BTV recombinant capripoxviruses (BTV-Cpox) individually expressing four different genes encoding two capsid proteins (VP2 and VP7) and two non-structural proteins (NS1, NS3) of BTV serotype 2 (BTV-2). Seroconversion was observed against NS3, VP7 and VP2 in both species and a lymphoproliferation specific to BTV antigens was also demonstrated in goats. Finally, partial protection of sheep challenged 3 weeks after BTV-Cpox administration with a virulent strain of BTV-2, was observed.
本文描述了用于反刍动物预防蓝舌病病毒（BTV）感染的重组山羊痘病毒的研制情况。用分别表达BTV血清型2（BTV-2）的两种衣壳蛋白（VP2和VP7）以及两种非结构蛋白（NS1、NS3）的四种不同基因的BTV重组山羊痘病毒（BTV-Cpox）对11只绵羊和4只山羊进行了免疫接种。在两个物种中均观察到针对NS3、VP7和VP2的血清转化，并且在山羊中也证实了对BTV抗原的特异性淋巴细胞增殖。最后，在用BTV-Cpox接种3周后用强毒株BTV-2攻击的绵羊中观察到了部分保护作用。
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