Key Laboratory of Grains and Oils Quality Control and Processing, Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering , Nanjing University of Finance and Economics , Nanjing 210023 , China.
School of Agriculture and Food Science , Meru University of Science and Technology , 972-60400 , Meru , Kenya.
J Agric Food Chem. 2019 Aug 14;67(32):8773-8782. doi: 10.1021/acs.jafc.9b02872. Epub 2019 Jul 23.
Conquering rapid postripeness and deterioration of is quite challenging. We previously observed that methyl jasmonate (MeJA) pretreatment postponed the deterioration of , but the mechanism is unknown. Here, a nontargeted metabolomics analysis by ultrahigh-pressure liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) revealed that MeJA increased the synthesis of malate by inhibiting the decomposition of fumarate and -aconitate. MeJA maintained energy supply by enhancing ATP content and energy charge level and improving hexokinase and glucose-6-phosphate dehydrogenase activities as well. These results promoted ATP supply by maintaining glycolysis, the TCA cycle, and the pentose phosphate pathway. In addition, we revealed that the delayed deterioration was attributed to MeJA treatment which stimulated the energy status of by reducing the respiration rate and nutrient decomposition, thus maintaining energy production. Our results provide a new insight into the role of MeJA treatment in delaying deterioration of through ATP production and supply.
克服荔枝的快速后熟和劣变是一项极具挑战性的任务。我们之前观察到茉莉酸甲酯（MeJA）预处理可以延缓荔枝的劣变，但具体机制尚不清楚。在此，通过超高压液相色谱-四极杆飞行时间串联质谱联用仪（UHPLC-QTOF-MS/MS）进行的非靶向代谢组学分析表明，MeJA 通过抑制富马酸和 - 柠康酸的分解来增加苹果酸的合成。MeJA 通过提高 ATP 含量和能量电荷水平，并增强己糖激酶和葡萄糖-6-磷酸脱氢酶的活性来维持能量供应。这些结果通过维持糖酵解、三羧酸循环和磷酸戊糖途径来促进 ATP 的供应。此外，我们还揭示了延迟劣变归因于 MeJA 处理，它通过降低呼吸速率和营养物分解来刺激荔枝的能量状态，从而维持能量产生。我们的研究结果为 MeJA 处理通过产生和供应 ATP 来延缓荔枝劣变提供了新的见解。
