Ohme M, Tanaka M, Chunwongse J, Shinozaki K, Sugiura M
FEBS Lett. 1986 May 5;200(1):87-90. doi: 10.1016/0014-5793(86)80516-6.
DNA sequencing has revealed a long open reading frame (ORF) in the large single-copy region of tobacco chloroplast DNA. This ORF consists of 1070 codons and its deduced amino acid sequence shows about 39% homology to that of the beta-subunit of E. coli RNA polymerase. This finding raises a possibility that some of the chloroplast RNA polymerase subunits are coded for by the chloroplast genome.
DNA测序揭示了烟草叶绿体DNA大单拷贝区域中的一个长开放阅读框(ORF)。这个ORF由1070个密码子组成,其推导的氨基酸序列与大肠杆菌RNA聚合酶β亚基的序列显示出约39%的同源性。这一发现增加了叶绿体RNA聚合酶的一些亚基由叶绿体基因组编码的可能性。