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本文引用的文献

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Submerged batch culture of the psychrophile Monographella nivalis in a defined medium; growth, carbohydrate utilization and responses to temperature.嗜冷菌雪腐单端孢霉在限定培养基中的深层分批培养；生长、碳水化合物利用及对温度的响应
New Phytol. 1995 Feb;129(2):299-308. doi: 10.1111/j.1469-8137.1995.tb04300.x.
2
Fructan Synthesis in Excised Barley Leaves (Identification of Two Sucrose-Sucrose Fructosyltransferases Induced by Light and Their Separation from Constitutive Invertases).离体大麦叶片中的果聚糖合成（两种光诱导的蔗糖-蔗糖果糖基转移酶的鉴定及其与组成型转化酶的分离）
Plant Physiol. 1993 Feb;101(2):459-468. doi: 10.1104/pp.101.2.459.
3
Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers of Helianthus tuberosus Colombia (II. Purification of Sucrose:Sucrose 1-Fructosyltransferase and Reconstitution of Fructan Synthesis in Vitro with Purified Sucrose:Sucrose 1-Fructosyltransferase and Fructan:Fructan 1-Fructosyltransferase).菊芋哥伦比亚品种块茎中果聚糖生物合成酶的纯化与特性分析（II. 蔗糖：蔗糖1-果糖基转移酶的纯化以及用纯化的蔗糖：蔗糖1-果糖基转移酶和果聚糖：果聚糖1-果糖基转移酶进行体外果聚糖合成的重组）
Plant Physiol. 1996 Apr;110(4):1167-1175. doi: 10.1104/pp.110.4.1167.
4
Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs.转基因马铃薯块茎积累高水平的蔗果三糖和蔗果四糖：菊芋（Cynara scolymus）花盘蔗糖-蔗糖 1-果糖基转移酶的功能鉴定。
Plant J. 1997 Nov;12(5):1057-65. doi: 10.1046/j.1365-313x.1997.12051057.x.
5
Fructan of the inulin neoseries is synthesized in transgenic chicory plants (Cichorium intybus L.) harbouring onion (Allium cepa L.) fructan:fructan 6G-fructosyltransferase.含有洋葱（葱属植物）果聚糖：果聚糖6G-果糖基转移酶的转基因菊苣植物（菊苣）可合成菊粉新系列果聚糖。
Plant J. 1997 Mar;11(3):387-98. doi: 10.1046/j.1365-313x.1997.11030387.x.
6
Synthesis of fructans in tubers of transgenic starch-deficient potato plants does not result in an increased allocation of carbohydrates.转基因淀粉缺乏型马铃薯植株块茎中果聚糖的合成不会导致碳水化合物分配增加。
Planta. 1996;199(4):528-36. doi: 10.1007/BF00195183.
7
Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus.通过组合来自菊芋块茎的纯化果糖基转移酶合成菊粉。
FEBS Lett. 1996 Apr 29;385(1-2):39-42. doi: 10.1016/0014-5793(96)00343-2.
8
Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley.蔗糖：果聚糖6-果糖基转移酶的纯化、克隆及功能表达，该酶是大麦中果聚糖合成的关键酶。
Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11652-6. doi: 10.1073/pnas.92.25.11652.
9
Purification and characterisation of an Aspergillus niger invertase and its DNA sequence.黑曲霉转化酶的纯化、特性鉴定及其DNA序列
Curr Genet. 1993 Jul-Aug;24(1-2):60-6. doi: 10.1007/BF00324666.
10
Characterization of levJ, a sucrase/fructanase-encoding gene from Actinomyces naeslundii T14V, and comparison of its product with other sucrose-cleaving enzymes.内氏放线菌T14V蔗糖酶/果聚糖酶编码基因levJ的特性分析及其产物与其他蔗糖裂解酶的比较
Gene. 1995 Jan 11;152(1):93-8. doi: 10.1016/0378-1119(94)00695-o.

在表达来自臭曲霉果糖基转移酶的转基因酵母中生产蔗果三糖。

Production of 1-kestose in transgenic yeast expressing a fructosyltransferase from Aspergillus foetidus.

作者信息

Rehm J, Willmitzer L, Heyer A G

机构信息

Max-Planck-Institut für Molekulare Pflanzenphysiologie, Golm, Germany.

出版信息

J Bacteriol. 1998 Mar;180(5):1305-10. doi: 10.1128/JB.180.5.1305-1310.1998.

DOI:10.1128/JB.180.5.1305-1310.1998
PMID:9495772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107021/
Abstract

Sucrose-inducible secretory sucrose:sucrose 1-fructosyltransferase (1-SST) from Aspergillus foetidus has been purified and subjected to N-terminal amino acid sequence determination. The enzyme is extensively glycosylated, and the active form is probably represented by a dimer of identical subunits with an apparent molecular mass of 180 kDa as judged from mobility in seminative acrylamide gels. The enzyme catalyzes fructosyl transfer from sucrose to sucrose producing glucose and 1-kestose. Oligosaccharides with a higher degree of polymerization are not obtained with sucrose as the substrate. The cDNA encoding the A. foetidus 1-SST has been cloned and sequenced. Sequence homology was found to be highest to levanases, but no hydrolytic activity was observed when levan was incubated with the enzyme. Expression of the cloned gene in an invertase-deficient mutant of Saccharomyces cerevisiae resulted in 1-kestose production, with 6-kestose and neokestose being side products of the reaction. Products were well distinguishable from those formed by yeast transformants expressing a cytosolic invertase.

摘要

从臭曲霉中纯化出蔗糖诱导型分泌性蔗糖

蔗糖1-果糖基转移酶（1-SST），并对其进行了N端氨基酸序列测定。该酶高度糖基化，根据在非变性丙烯酰胺凝胶中的迁移率判断，其活性形式可能是由相同亚基组成的二聚体，表观分子量为180 kDa。该酶催化果糖基从蔗糖转移到蔗糖，生成葡萄糖和1-蔗果三糖。以蔗糖为底物时，无法得到聚合度更高的寡糖。已克隆并测序了编码臭曲霉1-SST的cDNA。发现其与果聚糖酶的序列同源性最高，但将果聚糖与该酶一起孵育时未观察到水解活性。将克隆基因在酿酒酵母的转化酶缺陷型突变体中表达，可产生1-蔗果三糖，反应的副产物为6-蔗果四糖和新蔗果四糖。产物与表达胞质转化酶的酵母转化体形成的产物有明显区别。