United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi Matsuyama, Ehime 790-8566, Japan.
Fungal Biol. 2012 Jun;116(6):706-14. doi: 10.1016/j.funbio.2012.04.004. Epub 2012 Apr 22.
Sixty-two rotted wood and soil samples were used to screen for chrysene-degrading fungi. A strain of Fusarium, named F092, was identified as most capable of degrading chrysene. F092 was active under saline and nonsaline conditions, breaking down 48% of the chrysene in 30 d. The percentage of chrysene degraded did not change at 35‰ salinity with pH 8.2 in solid and liquid cultures. The degradation under saline conditions increased about 0.6- and 2.1-fold in cultures with polypeptone and Tween80, and 0.03-fold in agitated cultures. F092 secreted nonligninolytic enzymes named 1,2-dioxygenase and 2,3-dioxygenase. The level of 1,2-dioxygenase activity reached 203.5 U L(-1) at 30 d and that of 2,3-dioxygenase activity, 29.7 U L(-1) at 40 d. The degradation pathway was clarified from the intermediates produced; chrysene 1,2-oxide, chrysene trans-1,2-dihydrodiol, 1-hydroxy 2-naphtoic acid, and catechol. F092 is a potential degrader of chrysene for bioremediation.
采用 62 个腐朽木材和土壤样本筛选出能够降解䓛的真菌。一株被命名为 F092 的镰刀菌被鉴定为最有能力降解䓛的菌株。F092 在盐和非盐条件下都很活跃，在 30 天内可降解 48%的䓛。在 pH 值为 8.2、盐度为 35‰的固体和液体培养物中，䓛的降解率没有变化。在添加蛋白胨和吐温 80 的培养物中，盐度条件下的降解率分别增加了约 0.6 倍和 2.1 倍，在搅拌培养物中则增加了约 0.03 倍。F092 分泌了非木质素降解酶，分别命名为 1,2-加氧酶和 2,3-加氧酶。1,2-加氧酶的活性在 30 天时达到 203.5 U L(-1)，2,3-加氧酶的活性在 40 天时达到 29.7 U L(-1)。通过产生的中间产物阐明了降解途径：䓛 1,2-氧化物、䓛反-1,2-二氢二醇、1-羟基 2-萘甲酸和儿茶酚。F092 是一种潜在的用于生物修复的䓛降解菌。
