Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):965-970. doi: 10.1099/ijs.0.041186-0. Epub 2012 Jun 1.
A novel nitrogen-fixing strain, designated HL-12(T), was isolated from a freshwater lake in Taiwan. Cells of strain HL-12(T) were aerobic, Gram-negative, motile rods that were surrounded by a thick capsule, contained poly-β-hydroxybutyrate granules, and formed light-yellow to brownish-red colonies. Growth occurred at 15-40 °C (optimum 25-35 °C), at pH 6.0-7.0 (optimum pH 6.0) and with 0-4 % NaCl (optimum 0-1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HL-12(T) belonged to the genus Derxia and exhibited 99.1 and 98.8 % 16S rRNA gene sequence similarity, respectively, with Derxia gummosa IAM 14990 and D. gummosa IAM 13946(T). The major fatty acids (>10 %) of strain HL-12(T) were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c. The cellular hydroxy fatty acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH. The isoprenoid quinone was Q-8 and the DNA G+C content was 72.0 mol%. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several unknown aminophospholipids and phospholipids. DNA-DNA relatedness between strain HL-12(T) and http://dx.doi.org/10.1601/nm.1758 LMG 3975 and http://dx.doi.org/10.1601/nm.1758 LMG 3977(T) was <70 %. On the basis of the genotypic and phenotypic data, strain HL-12(T) represents a novel species in the genus Derxia, for which the name Derxia lacustris sp. nov. is proposed. The type strain is HL-12(T) ( = BCRC 80208(T) = KCTC 23311(T)).
一株新型固氮菌株，命名为 HL-12(T)，从台湾的一个淡水湖中分离得到。HL-12(T)菌株的细胞为好氧、革兰氏阴性、能动的杆状菌，周围有一层厚厚的荚膜，含有多聚β-羟基丁酸颗粒，并形成浅黄色到红棕色的菌落。生长温度为 15-40°C（最佳温度为 25-35°C），pH 值为 6.0-7.0（最佳 pH 值为 6.0），盐度为 0-4%（最佳盐度为 0-1%）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HL-12(T)属于 Derxia 属，与 Derxia gummosa IAM 14990 和 D. gummosa IAM 13946(T)的 16S rRNA 基因序列相似度分别为 99.1%和 98.8%。菌株 HL-12(T)的主要脂肪酸（>10%）为 sum feature 3（由 C16:1ω7c 和/或 C16:1ω6c、C16:0 和 C18:1ω7c 组成）、C16:0 和 C18:1ω7c。细胞中的羟基脂肪酸为 C12:0 3-OH、C14:0 2-OH 和 C14:0 3-OH。异戊烯醌为 Q-8，DNA G+C 含量为 72.0 mol%。极性脂谱含有磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和几种未知的氨基磷脂和磷脂。菌株 HL-12(T)与 http://dx.doi.org/10.1601/nm.1758 LMG 3975 和 http://dx.doi.org/10.1601/nm.1758 LMG 3977(T)的 DNA-DNA 同源性<70%。基于基因型和表型数据，菌株 HL-12(T)代表 Derxia 属中的一个新型物种，建议将其命名为 Derxia lacustris sp. nov.。模式菌株为 HL-12(T)（=BCRC 80208(T)=KCTC 23311(T)）。
