Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3755-3761. doi: 10.1099/ijs.0.050609-0. Epub 2013 May 3.
A novel nitrogen-fixing strain, designated DQS-4(T), was isolated from oil-contaminated soil in Taiwan and was characterized using a polyphasic taxonomic approach. Cells of strain DQS-4(T) stained Gram-negative, contained poly-β-hydroxybutyrate granules and were motile rods, surrounded by a thin capsule. Cells displayed a strictly aerobic type of metabolism and fixed nitrogen microaerobically. Growth occurred at 10-45 °C (optimum, 35-40 °C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-2 % NaCl (optimum, 0.5-1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DQS-4(T) belonged to the genus Azoarcus, and its closest neighbours were Azoarcus indigens VB32(T) and Azoarcus communis SWub3(T), with sequence similarities of 97.4 and 96.4 %, respectively. The major cellular fatty acids of strain DQS-4(T) were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c. The major cellular hydroxy fatty acid was C10 : 0 3-OH. The DNA G+C content was 64.5 mol%. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The mean level of DNA-DNA relatedness between strain DQS-4(T) and A. indigens LMG 9092(T) was 27.4 %. On the basis of the genotypic and phenotypic data, strain DQS-4(T) represents a novel species in the genus Azoarcus, for which the name Azoarcus olearius sp. nov. is proposed. The type strain is DQS-4(T) ( = BCRC 80407(T) = KCTC 23918(T) = LMG 26893(T)).
一株新的固氮菌株，命名为 DQS-4(T)，从台湾受污染的土壤中分离得到，并通过多相分类学方法进行了表征。DQS-4(T) 菌株的细胞呈革兰氏阴性，含有多聚-β-羟基丁酸颗粒，是运动的杆状菌，周围有一层薄的荚膜。细胞表现出严格需氧的代谢类型，并微需氧固定氮。生长发生在 10-45°C（最佳，35-40°C），在 pH 7.0-8.0（最佳，pH 7.0）和 0-2%NaCl（最佳，0.5-1%）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 DQS-4(T) 属于固氮单胞菌属，其最接近的亲缘种是 Azoarcus indigens VB32(T)和 Azoarcus communis SWub3(T)，序列相似度分别为 97.4%和 96.4%。DQS-4(T) 菌株的主要细胞脂肪酸是组成 3 的总和（包括 C16:1ω7c 和/或 C16:1ω6c）、C16:0 和 C18:1ω7c。主要的细胞羟基脂肪酸是 C10:0 3-OH。DNA G+C 含量为 64.5 mol%。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和几种未鉴定的氨基磷脂和磷脂组成。DQS-4(T) 菌株与 A. indigens LMG 9092(T) 之间的 DNA-DNA 相关平均水平为 27.4%。基于基因型和表型数据，菌株 DQS-4(T) 代表固氮单胞菌属中的一个新种，建议命名为 Azoarcus olearius sp. nov.。模式菌株为 DQS-4(T)（=BCRC 80407(T)=KCTC 23918(T)=LMG 26893(T)）。
