Fennimore S A, Nyquist W E, Shaner G E, Doerge R W, Foley M E
Department of Vegetable Crops, University of California at Davis, c/o USDA-ARS, 1636 East Alisal St, CA 93905-3018, Salinas, USA, US,
Theor Appl Genet. 1999 Aug;99(3-4):711-8. doi: 10.1007/s001220051288.
Seed dormancy allows weed seeds to persist in agricultural soils. Wild oat (Avena fatua L.) is a major weed of cereal grains and expresses a range of seed dormancy phenotypes. Genetic analysis of wild oat dormancy has been complicated by the difficulty of phenotypic classification in segregating populations. Therefore, little is known about the nature of the genes that regulate dormancy in wild oat. The objectives of our studies were to develop methods to classify the germination responses of segregating wild oat populations and to find molecular markers linked to quantitative trait loci (QTL) that regulate seed dormancy in wild oat. RAPD markers OPX-06 and OPT-04 explained 12.6% and 6.8% respectively, of the F(2) phenotypic variance. OPF-17 was not significant in a simple regression model, but it was linked in repulsion to OPT-04. A three-locus model of seed dormancy in wild oat is presented based on the 41-day germination profiles of F(1), F(2), F(3), BC(1)P(1)F(1), BC(1)P(1)F(2), and BC(1)P(2)F(1) generations, and the 113 day germination profile of 126 F(7) recombinant inbred lines. Loci G (1) and G (2) promote early germination, and the D locus promotes late germination. If at least one copy of the dominant G (1) or G (2 )alleles are present regardless of the genotype at D locus, then the individual will be nondormant. If the genotype is g (1) g (1) g (2) g (2) D_, then the phenotype will be dormant.
种子休眠使杂草种子能在农业土壤中持续存在。野燕麦（Avena fatua L.）是谷物的主要杂草，表现出一系列种子休眠表型。由于在分离群体中进行表型分类存在困难，野燕麦休眠的遗传分析变得复杂。因此，对于调控野燕麦休眠的基因本质了解甚少。我们研究的目的是开发方法来分类分离的野燕麦群体的萌发反应，并找到与调控野燕麦种子休眠的数量性状位点（QTL）连锁的分子标记。RAPD标记OPX - 06和OPT - 04分别解释了F(2)表型变异的12.6%和6.8%。OPF - 17在简单回归模型中不显著，但它与OPT - 04呈相斥连锁。基于F(1)、F(2)、F(3)、BC(1)P(1)F(1)、BC(1)P(1)F(2)和BC(1)P(2)F(1)代的41天萌发曲线，以及126个F(7)重组自交系的113天萌发曲线，提出了一个野燕麦种子休眠的三位点模型。位点G (1)和G (2)促进早期萌发，D位点促进晚期萌发。如果无论D位点的基因型如何，至少存在一个显性G (1)或G (2)等位基因的拷贝，那么个体将是非休眠的。如果基因型是g (1) g (1) g (2) g (2) D_，那么表型将是休眠的。
