Nikolov Miroslav, Schmidt Carla, Urlaub Henning
Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany.
Methods Mol Biol. 2012;893:85-100. doi: 10.1007/978-1-61779-885-6_7.
In recent years, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review the strategies and problems of the methods currently in use for the quantitative analysis of peptides, proteins, and finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.
近年来，质谱技术比以往任何时候都更深入地进入了以蛋白质为中心的研究前沿。在定性层面确立之后，使用质谱技术对蛋白质和肽进行定量这一更具挑战性的问题已成为进一步发展的焦点。在本章中，我们讨论并回顾了目前用于通过质谱对肽、蛋白质乃至蛋白质组进行定量分析的方法的策略和问题。介绍了主要方法的共同主题、差异及潜在陷阱，以便对基于质谱的定量蛋白质组学这一新兴领域进行综述。
