Li Jianqing, Lu Qianyi, Lu Peirong
Department of Ophthalmology, the First Affiliated Hospital of Soochow University, No. 188 Shizi street, Suzhou, 215006, China.
BMC Ophthalmol. 2018 Jun 26;18(1):151. doi: 10.1186/s12886-018-0821-3.
To compare the abundance of vitreous proteins between the patients with proliferative diabetic retinopathy (PDR) and idiopathic macular hole (IMH).
In this study, we performed mass spectrometry-based label-free quantitative proteomics analysis of vitreous samples from type 2 diabetic patients with PDR (n = 9) and IMH subjects (n = 9) and identified the abundance of 610 proteins.
Out of 610 proteins, 64 proteins (Group A) were unique to PDR patients, while 212 proteins (Group B) could be identified in IMH vitreous only. Among the other 334 proteins that could be detected in both PDR and IMH eyes, 62 proteins differed significantly (p < 0.05, fold change > 2), which included 52 proteins (Group C) and 10 proteins (Group D) over- and under-expressed in PDR vitreous compared with the control. All proteins in these four groups were counted as significant proteins in our study.
We identified and quantified 610 proteins in total, which included 338 significant proteins in our study. Protein distribution analysis demonstrated a clear separation of protein expression in PDR and IMH. The protein function analysis illustrated that immunity and transport related proteins might be associated with PDR.
比较增殖性糖尿病视网膜病变（PDR）患者和特发性黄斑裂孔（IMH）患者玻璃体蛋白质的丰度。
在本研究中，我们对2型糖尿病PDR患者（n = 9）和IMH患者（n = 9）的玻璃体样本进行了基于质谱的无标记定量蛋白质组学分析，共鉴定出610种蛋白质的丰度。
在610种蛋白质中，64种蛋白质（A组）仅在PDR患者中存在，而212种蛋白质（B组）仅在IMH玻璃体中可检测到。在PDR和IMH眼中均可检测到的其他334种蛋白质中，有62种蛋白质存在显著差异（p < 0.05，倍数变化> 2），其中与对照组相比，PDR玻璃体中有52种蛋白质（C组）表达上调，10种蛋白质（D组）表达下调。在我们的研究中，这四组中的所有蛋白质均被视为显著蛋白质。
我们总共鉴定并定量了610种蛋白质，其中在我们的研究中有338种显著蛋白质。蛋白质分布分析表明PDR和IMH中的蛋白质表达有明显差异。蛋白质功能分析表明，免疫和转运相关蛋白质可能与PDR有关。
