Honda K, Harihara S, Nakamura T, Hirai M, Misawa S
Department of Legal Medicine, University of Tsukuba, Japan.
Nihon Hoigaku Zasshi. 1990 Aug;44(4):293-301.
Sex identification of unidentified two bleached skeletons by the method of DNA analysis in addition to the morphological examination was performed. Bones and teeth were taken out for the DNA analysis, and cracked to compounds by liquid nitrogen. DNA was extracted by the standard procedure. We used two methods for sex identification by DNA analysis; one is Southern blot hybridization with Y-chromosome specific probe (PHY10; 3.4kb), and the other is PCR (polymerase chain reaction) amplifying with sex chromosome specific fragments (alphoid satellite family). These two methods enable us to identify the sex of the two cases presented here more correctly with sufficient evidence than depending only on morphological examination. With some technical difficulties, it is possible to put DNA analysis including Southern blot hybridization or PCR into practical determination of sexing of hard tissues such as a piece of bone or a tooth, and old samples. Therefore, they seem to be effective methods applicable to forensic science.
除形态学检查外，还采用DNA分析方法对两具身份不明的白骨进行了性别鉴定。取出骨骼和牙齿进行DNA分析，并用液氮将其破碎成小块。按照标准程序提取DNA。我们采用两种DNA分析方法进行性别鉴定；一种是用Y染色体特异性探针（PHY10；3.4kb）进行Southern印迹杂交，另一种是用性染色体特异性片段（α卫星家族）进行PCR（聚合酶链反应）扩增。与仅依靠形态学检查相比，这两种方法能让我们更准确地鉴定此处所呈现的两例案件的性别，且有充分的证据。尽管存在一些技术难题，但将包括Southern印迹杂交或PCR在内的DNA分析应用于诸如一块骨头或一颗牙齿等硬组织以及陈旧样本的实际性别鉴定是有可能的。因此，它们似乎是适用于法医学的有效方法。
