Tiwari Sudhanshu, Tiwari Richa, Singh Ajay
Natural Product Laboratory, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur 273009, India.
ScientificWorldJournal. 2012;2012:291395. doi: 10.1100/2012/291395. Epub 2012 Apr 30.
Laboratory evaluations were made to assess the toxicological and biochemical effect of cypermethrin on fingerlings of common edible freshwater culture carp (Labeo rohita). There was a significant negative (P < 0.05) correlation observed between effective doses of cypermethrin and exposure periods; that is, LC₅₀ values decreased from 0.323 μg/L (6 h) to > 0.278 μg/L (12 h), > 0.240 μg/L (18 h) and >0.205 μg/L (24 h). Exposure to sublethal doses of cypermethrin for 24 h and 96 h exposure period caused significant (P < 0.05) time- and dose-dependent alterations in total protein, total free amino acids, nucleic acids, glycogen, pyruvate, and lactate level and in the activity of enzyme protease, alanine aminotransferase, aspartate aminotransferase, acid phosphatases, alkaline phosphatases, acetylcholinesterase, and cytochrome oxidase in liver and muscle tissues of fish. Thus, cypermethrin has potent piscicidal activity against fingerlings of fish Labeo rohita and adversely affects their behavioural patterns, shifting aerobic pathway of fish respiration towards anaerobic pathway and also inhibiting energy production by suppressing ATP synthesis.
进行了实验室评估，以评估氯氰菊酯对常见食用淡水养殖鲤鱼（印度鲤）幼鱼的毒理学和生化影响。观察到氯氰菊酯的有效剂量与暴露时间之间存在显著的负相关（P < 0.05）；也就是说，半数致死浓度（LC₅₀）值从0.323μg/L（6小时）降至>0.278μg/L（12小时）、>0.240μg/L（18小时）和>0.205μg/L（24小时）。在24小时和96小时的暴露期内，接触亚致死剂量的氯氰菊酯会导致鱼类肝脏和肌肉组织中的总蛋白、总游离氨基酸、核酸、糖原、丙酮酸和乳酸水平以及蛋白酶、丙氨酸转氨酶、天冬氨酸转氨酶、酸性磷酸酶、碱性磷酸酶、乙酰胆碱酯酶和细胞色素氧化酶的活性出现显著（P < 0.05）的时间和剂量依赖性变化。因此，氯氰菊酯对印度鲤幼鱼具有强大的杀鱼活性，并对其行为模式产生不利影响，使鱼类呼吸的有氧途径转向无氧途径，还通过抑制ATP合成来抑制能量产生。
